鸭出血性卵巢炎毒对产蛋鸭的致病性研究.pdfVIP

摘 要 本研究从生产实际出发，主要从病理学方面研究 2010 年 4 月份以来致使产蛋 鸭产蛋量急剧下降的新发流行病—鸭出血性卵巢炎。研究目的是通过病理学分析鸭 出血性卵巢炎发病鸭的病理变化、抗原在机体内的分布，并建立鸭出血性卵巢炎对 不同日龄产蛋鸭的人工感染模型，为该病以后的防制提供实验依据。 实验方法：用北京农林科学院畜牧兽医研究所繁殖的DHO-F4 代病毒液分别感 染350d 和500d 的产蛋鸭，在攻毒后在第4 、5、6d 时每组随机抽取一或两只产蛋 鸭扑杀，采血，分离血清，同时采取攻毒产蛋鸭的咽拭子和肛拭子接种鸡胚，并进 行尸体剖检，采集卵巢、脾脏等组织进行病毒分离，其余系统取材，进行病理组织 学检查和病毒抗原的免疫组化染色。 实验结果：DHOV 人工感染350d 和500d 的产蛋鸭后，产蛋鸭出现卵泡变性、 变形、出血，脾脏肿大、大脑轻度水肿等特征性病变，符合自然病例特征。免疫组 化结果表明：抗原主要存在于大脑的小胶质细胞，脾脏的淋巴细胞，肝脏的肝细胞， 肾脏的肾小管上皮细胞及卵泡膜细胞。并且攻毒剂量越大，产蛋鸭出现的病理现象 越明显，免疫组化阳性反应越强。 结论：鸭出血性卵巢炎病毒可以通过人工途径成功感染产蛋鸭，并能致使攻毒 后的产蛋鸭产生出血性卵巢炎及各组织脏器出现间质性增生等特征性病变。免疫组 化结果表明：病毒主要攻击脾脏、卵泡膜、大脑、肝脏等组织器官。 关键词：鸭出血性卵巢炎；病毒；病理组织学；抗原定位 Pathogenicity Studies of Duck Hemorrhagic Ovarian Virus on the Ducks Abstract The experiment was conducted to a new epidemic attacked on the ducks and which can cause the ducks to get a sharp decline – named as duck hemorrhagic ovaritis, DHO. The main purpose of this experiment is to conduct the pathological analysis of the pathological changes of duck hemorrhagic ovarian minflammation incidence duck antigens in the body distribution, and to create artificial infection model duck hemorrhagic ovarian inflammation on different days of age ducks, and also provide a reliable reference for advice on disease prevention and control for future. Test methods: In this experiment, we used the normal negative production ducks as the object of study, and used the DHO-F4 generation of virus solution which was breeded by the Institute of Animal, belongs to Beijing Academy of Agiculture and Forestry Sciences, to attack ducks. After that, we should observe and record the incidence of the ducks. After each batch of ducks were inoculated in the firs