25S rDNA和5S rDNA在大白菜中期染色体上的FISH定位.pdfVIP

中国农业科学 2007,40(4):782-787 Scientia Agricultura Sinica 25S rDNA和5S rDNA在大白菜中期染色体上的FISH定位 轩淑欣，申书兴，赵建军，张成合，陈雪平，郄丽娟 （河北农业大学园艺学院，保定071001 ） 摘要：【目的】确定rDNA在中国大白菜基因组中的位点数目和分布位置，并建立识别大白菜不同染色体的特 异标记。【方法】用荧光原位杂交技术对25S rDNA和5S rDNA在大白菜有丝分裂中期染色体进行了定位研究。【结 果】在大白菜中期染色体上，分别检出 了5对25S rDNA 杂交信号和3 对5S rDNA杂交信号。对应于大白菜中期染 色体形态图，确定5对25S rDNA信号分别分布在大白菜1 号染色体长臂（1L）的近末端，2号染色体长臂（2L） 的近中部，3号和4号染色体长臂（3L、4L）的近着丝点处和10号染色体的随体上，信号强度为10 号2号3号 和4号1号；而5S rDNA的3 对信号分别位于2号染色体的长臂（2L）和9号、10号染色体的短臂（9S、10S）。 【结论】在分子水平上为大白菜部分染色体提供了识别标记。 关键词：大白菜；25SrDNA；5S rDNA；荧光原位杂交 Location of 25S rDNA and 5S rDNA in Chinese Cabbage-pe-tsai Metaphase Chromosome XUAN Shu-xin, SHEN Shu-xing, ZHAO Jian-jun, ZHANG Cheng-he, CHEN Xue-ping, QIE Li-juan (College of Horticulture, Hebei Agricultural University, Baoding 071001) Abstract: 【Objective 】The objective was to determine the number and location of rDNA sites in Chinese cabbage-pe-tsai genome, and identify the special molecular markers related to different chromosomes. 【Method 】Fluorescence in situ hybridization(FISH) technique was used to locate the 25S rDNA and 5S rDNA on the mitotic metaphase chromosomes of Chinese cabbage-pe-tsai. 【Result 】Five pairs of hybridization signals of 25S rDNA and three pairs of hybridization signals of 5S rDNA were detected on the metaphase chromosomes. Contrasting the normal karyotype of the diploid Chinese cabbage-pe-tsai, the 25S rDNA hybridization signals were located near the end of 1L, the central section of 2L, the centromere region of 3L and 4L, and the satellite of chromosome 10, respectively. The intensity of hybridization signals was chromosome 10 chromosome 2 chromosome 3 and chromosome 4 chromosome 1. Hybridization signals of 5S rDNA were located on 2L, 9S and 10S. 【Conclusion 】These re