大豆异黄酮对金黄色葡萄球菌的抑菌机制研究.pdfVIP

中国农业科学 2009,42(7):2586-2591 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.07.041 大豆异黄酮对金黄色葡萄球菌的抑菌机制研究 王海涛，王 倩，谢明杰 （辽宁师范大学生命科学学院，大连 116029 ） 摘要：【目的】研究大豆异黄酮（Soybean isoflavone，SI）的抑菌机制。【方法】本试验利用呼吸代谢抑制 实验和 4,6-二脒基-2-苯基吲哚（4,6-diamidino-2-phenylindole，DAPI）荧光染色对大豆异黄酮的抑菌机制 进行研究。【结果】呼吸代谢抑制实验结果表明，当 SI 的终浓度为 0.8 mg·ml-1 时，可显著抑制金黄色葡萄球菌苹 果酸脱氢酶的活性，SI 组苹果酸脱氢酶的比活性与对照组相比降低了 74.97%。DAPI 染色结果显示，DAPI 可以和 金黄色葡萄球菌内的核酸结合，经过 SI 作用 28 h 后，菌体内的核酸含量呈明显下降趋势，其中 DNA 的荧光强度 减少了 33.53%，RNA 减少了 39.82%。【结论】SI 具有有较强的抑菌活性，其抑菌机制是通过抑制细菌的呼吸代谢 和核酸的合成等方面实现的。 关键词：大豆异黄酮；抑菌机制；苹果酸脱氢酶；DAPI Anti-Bacterial Mechanism of Soybean Isoflavone on Staphylococcus aureus WANG Hai-tao, Wang Qian, XIE Ming-jie (The College of Life Science, Liaoning Normal University, Dalian 116029) Abstract: 【Objective 】 To investigate the anti-bacterial mechanism of soybean isoflavone (SI) and provide a theoretical basis for the development of high-performance and low toxicity of antibacterial drugs. 【Method 】 Respiratory metabolism test and 46-diamidino-2-phenylindole (DAPI) staining assay were used to investigate the antibacterial mechanism of SI. 【Result 】 The results indicated that the malic dehydrogenase (MDH) activity of Staphylococcus aureus was significantly inhibited by 0.8 mg·ml-1 SI and the malic dehydrogenase (MDH) activity of Staphylococcus aureus was remarkably reduced by 74.97% compared to the control group. The fluorescence microscopy and fluorescence spectrophotometer studies manifested that 46-diamidino-2- phenylindole (DAPI) could integrate with Staphylococcus aureus, and the quant