促孕散对不孕奶牛血清中微量元素和白介素的影响.pdfVIP

中国农业科学 2012,45(13):2758-2763 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.13.021 促孕散对不孕奶牛血清中微量元素和白介素的影响 邓 敏，谷新利，梁光华，刘贤侠，高 树，徐 雷 （石河子大学动物科技学院，新疆石河子，832003 ） 摘要：【目的】探讨经促孕散对不孕奶牛治疗前后血清中微量元素（Cu、Fe、Mn、Zn）与白介素（IL-1、IL-6） 的含量变化规律。【方法】利用火焰法、原子吸收光谱法和酶联免疫吸附法检测实验组和正常组治疗前、后血清中 微量元素和白介素的含量。【结果】①促孕散治疗持久，黄体有效率为 91.67%，卵巢静止有效率为 88.89%，②持 久黄体型治疗前血清中Cu、Zn含量显著高于正常组（P＜0.05），Mn含量极显著低于正常组（P＜0.01），治疗后血清 中含量接近正常，且含量水平差异不显著（P＞0.05），Fe、IL-1、IL-6含量治疗前后与正常组比较差异不显著，③ 卵巢静止型治疗前血清中Mn含量低于正常组（P＜0.01），IL-1含量高于正常组（P＜0.05），治疗后逐步与正常组含 量水平差异不显著（P＞0.05），Cu、 Fe、Zn、IL-6含量治疗前后与正常组比较差异不显著（P＞0.05）。【结论】促 孕散对不孕奶牛血清中部分微量元素和白介素含量有微调作用，在奶牛不孕症治疗效果上有不可忽视的作用。 关键词：促孕散；奶牛；持久黄体；卵巢静止；血清；微量元素；白介素 Effects of Fertility-Promoting Powder on Trace Elements and Interleukin in Serum in Infertile Dairy Cattle DENG Min ，GU Xin-li，LIANG Guang-hua ，LIU Xian-xia ，GAO Shu，XU Lei (College of Animal Science Technology, Shihezi University, Shihezi 832003, Xinjiang) Abstract: 【Objective 】The law of trace elements (Cu, Fe, Mn, Zn) and interleukin (IL-1, IL-6) contents in serum using fertility-promoting powder to treat infertility caused by ovarian disease of dairy cattle were studied. 【Method 】 Flame atomic absorption spectrometry and enzyme-linked immunization absorb assay were used to detect the levels of trace elements and interleukin contents in serum from the experimental and normal groups before and after treatment. 【Result 】 After treatment, the effective rate of persistent corpus luteum was 91.67%, the effective rate of ovarian static was 88.89%. In persistent corpus luteum body, Cu, Zn contents were significantly higher than normal group in pre-treatment (P ＜0.05)