大白菜-结球甘蓝5号二体异附加系的选育及鉴定.pdfVIP

中国农业科学 2010,43(14):2937-2943 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.14.012 大白菜-结球甘蓝5号二体异附加系的选育及鉴定 孟雅宁，王彦华，顾爱侠，李晓峰，申书兴 （河北农业大学园艺学院，河北保定 071000 ） 摘要：【目的】获得稳定遗传的大白菜-结球甘蓝二体异附加系。【方法】以大白菜-结球甘蓝5号单体异附加 系为材料，对其自交后代进行细胞学及 SSR 鉴定。【结果】37 株自交后代中 2n=22 植株的比率为 10.81%，通过对 4个2n=22的植株进行核型分析及SSR鉴定，确定其为大白菜-结球甘蓝5号二体异附加系。减数分裂观察发现， 大白菜-结球甘蓝 5 号二体异附加系后期Ⅰ虽然出现染色体落后现象，但在后期Ⅱ中染色体的分裂方式以 11-11- 11-11 为主，其比率为 69.55%。【结论】获得了大白菜-结球甘蓝 5 号二体异附加系，其外源染色体与甘蓝 07 连 锁群相对应；该二体异附加系减数分裂形成的四分体以附加 1 条的染色体为主（n=11），有较高的遗传稳定性。 二体异附加系的获得为研究芸薹属 A、C 基因组的亲缘关系，以及向大白菜中导入结球甘蓝的优良基因具有重要 意义。 关键词：大白菜；结球甘蓝；核型分析；分子标记；二体异附加系 Generation and Identification of 5# Chinese Cabbage-Cabbage Disomic Alien Addition Lines MENG Ya-ning, WANG Yan-hua, GU Ai-xia, LI Xiao-feng, SHEN Shu-xing (College of Horticulture, Agricultural University of Hebei, Baoding 071000, Hebei) Abstract: 【Objective 】 The objective of this study is to obtain homozygous Chinese cabbage-cabbage disomic alien addition lines. 【Method 】 In this study, 5#Chinese cabbage-cabbage monosomic addition line was used, and its selfing F generation was 1 tested by means of identification of cytology and SSR analysis. 【Result 】 The results showed that the ratio of disomic addition lines (2n=22) was 10.81% in 37 screened plants of selfed progeny. Through karyotype analysis and molecular marker identification, 4 Chinese cabbage-cabbage disomic alien addition lines (Chinese cabbage with 5# chromosome from cabbage) were identified in the plants with 2n=22. The alien chromosomes of disomic alien addition lines moving late was obs