大豆主要农艺性状的QTL分析.pdfVIP

中国农业科学 2007,40(1):41-47 Scientia Agricultura Sinica 大豆主要农艺性状的QTL分析 陈庆山，张忠臣，刘春燕，辛大伟，单大鹏，邱红梅，单彩云 （东北农业大学大豆研究所，哈尔滨 150030 ） 摘要：【目的】大豆的多数农艺性状均为重要的数量性状。对大豆的数量性状进行基因定具有重要的研究和 应用价值。【方法】以美国半矮秆大豆品种Charleston为母本，东北农业大学高蛋白大豆品系东农594为父本及 其F2:10代重组自交系的154个株系为实验材料。164个SSR引物经亲本筛选后用于群体扩增，并构建遗传图谱。对 亲本间表现多态的12个农艺性状进行了调查及QTL分析。【结果】农艺性状包括品质性状（蛋白质含量、油分含 量、蛋脂总量等）；产量性状（单株荚数、单株粒重、百粒重等）和其它农艺性状（株高、生育期、分枝数、主茎 节数、平均叶长、平均叶宽等）。结果表明，12个农艺性状共检出68个QTLs。每个性状的QTLs检出个数从平均 叶宽的3个到百粒重、株高等的8个，平均每个性状检测出5.8个。与国内外对应农艺性状QTL检测结果相比， 多个性状的QTL位点均一致，说明QTL检测准确率较高，可以进一步用于分子辅助育种。【结论】获得了大豆12 个重要农艺性状的68个主效QTLs。 关键词：大豆；农艺性状；QTL；SSR；分析 QTL Analysis of Major Agronomic Traits in Soybean CHEN Qing-shan, ZHANG Zhong-chen, LIU Chun-yan, XIN Da-wei, SHAN Da-peng, QIU Hong-mei, SHAN Cai-yun (Soybean Research Institute, Northeast Agricultural University, Haerbin 150030) Abstract: 【Objective 】Soybean is a major crop, whose most agronomic traits are quantitative. Locating these quantitative trait loci in soybean genes has strong application values. 【Method 】A F2:10 RIL population containing 154 lines derived from cross between Charleston as female and Dongnong 594 as male parent were used in this experiment. A genetic linkage map was constructed with 164 SSR primers screened in two parents and amplified in the 154 line population. 12 agronomic traits showing polymorphic in parents were investigated and QTLs were analyzed. QTLs of all the traits were analyzed with software Windows QTL Cartographer V2.0. 【Result 】The agronomic traits included the following quality traits: protein content, oil content, content of protein and oil; yield traits: pods per plant, seed weight per plant, 100 seeds weight; and other a