刀孢蜡蚧菌的鉴定及其对南方根结线虫不同生活阶段的定殖.pdfVIP

中国农业科学 2012,45(12):2404-2411 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.12.008 刀孢蜡蚧菌的鉴定及其对南方根结线虫不同生活阶段的定殖 曹君正，武 侠，林 森 （青岛农业大学农学与植物保护学院/ 山东省植物病虫害综合防控重点实验室，山东青岛266109 ） 摘要：【目的】明确卵寄生真菌（Lecanicillium psalliotae ）刀孢蜡蚧菌对根结线虫的防治潜力。【方法】 采用分离自山东青岛城阳丝瓜根结线虫的卵寄生真菌 CGMCC5329，通过形态学特征观测及分子生物学分析，鉴定 该菌株为刀孢蜡蚧菌。显微观察刀孢蜡蚧菌对南方根结线虫（Meloidogyne incognita ）不同生活阶段的定殖。【结 果】刀孢蜡蚧菌能够寄生南方根结线虫生活史中的卵、2 龄幼虫和雌虫，同时对卵块内卵孵化具有明显的抑制作 用。该菌菌丝在卵表面形成侵染钉，卵壳皱缩凹陷，卵胚胎发育停止和内容物凝集，卵内长满菌丝，有时可见到 菌丝上形成瓶梗细胞和分生孢子。第6 天卵寄生率为85.76%；15 d 卵块相对孵化抑制率为91.41%。被真菌寄生 的2 龄幼虫体表形成致密菌网，穿透体壁，体内长满菌丝，5 d 后寄生率为79.23%。寄生的雌虫体壁形成致密菌 网和侵染钉，体壁皱缩变形，有时内容物外渗。【结论】刀孢蜡蚧菌CGMCC5329 不仅能寄生根结线虫生活史中定居 态的雌虫和卵，还能寄生侵染态2 龄幼虫。该菌是具有生物防治应用潜力的根结线虫寄生真菌。 关键词：刀孢蜡蚧菌；南方根结线虫；ITS 序列；系统发育；致病性 Identification of Fungus Lecanicillium psalliotae and Its Colonization in Different Life Stages of Meloidogyne incognita CAO Jun-zheng, WU Xia, LIN Sen (College of Agronomy and Plant Protection, Qingdao Agricultural University/Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, Shandong) Abstract: 【Objective 】 The objective of this study is to investigate the control potential of egg-parasitic fungi Lecanicillium psalliotae against root-knot nematodes as an useful biological control agent. 【Method 】The fungus strain CGMCC5329 was isolated from eggs of Meloidogyne incognita infecting sponge gourd in Chengyang, Qingdao, Shandong Province. Based on morphological characters and molecular analysis of ITS-rDNA, the strain was identified as L. psalliotae . Colonization of L. psalliotae in different life stages of M. incognita was observed by microscope. 【Result 】The fungus colonized in different life stages of M ．incognita includ