大球盖菇多糖的分子质量分布及其单糖的组成.pdfVIP

中国农业科学 2011,44(10):2109-2117 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.10.016 大球盖菇多糖的分子质量分布及其单糖的组成 陈君琛，翁敏劼，赖谱富，李怡彬，周学划，沈恒胜 （福建省农业科学院农产品加工研究中心/福建省农业科学院农业工程技术研究所，福州 350003 ） 摘要：【目的】从大球盖菇(Stropharia rugoso-annulata)中提取多糖，并对其分子质量及组成糖进行研究。 【方法】采用水提法提取大球盖菇多糖，利用高效液相色谱法研究大球盖菇多糖分子质量分布及提取过程中多糖 分子质量的变化，并采用红外光谱、核磁共振法对其组成糖进行研究。【结果】大球盖菇多糖水溶性多糖类物质的 分子质量分布于22 kD 附近；大球盖菇多糖同时存在α和β糖苷键，以吡喃糖为主，由5 种单糖构成，其中有D- 果糖、D-葡萄糖和 D-木糖。【结论】以稀盐溶液为流动相的检测手段更能真实地反映大球盖菇多糖的分子质量分 布情况，大球盖菇多糖是由5 种单糖组成的杂多糖。 关键词：大球盖菇；多糖；分子质量 Distribution of Stropharia rugoso–annulata Polysaccharides Molecular Weight and Component Sugar CHEN Jun-chen, WENG Min-jie, LAI Pu -fu , LI Yi-bin, ZHOU Xue-hua, SHEN Heng-sheng (Agricultural Product Processing Research Centre, Fujian Academy of Agricultural Sciences/ Research Institute of Agri-Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003) Abstract: 【Objective 】The aim of this paper was to extract and isolate Stropharia rugoso-annulata polysaccharides and analyze the molecular weight and component sugar of polysaccharides. 【Method 】In this paper, polysaccharide from Stropharia rugoso-annulata was obtained by water extraction method, which the molecular weight distribution and changes during extraction course was investigated by using high performance liquid chromatography. The component sugar of the polysaccharides were also measured by infrared spectrum and nuclear magnetic resonance methods. 【Result 】The molecular weight distribution of water soluble polysaccharides from Stropharia rugoso-annulata was about 22 kD, α and β glucosidic bonds were co-exist in polysaccharides molecule which contain 5 kinds of mon