茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用.pdfVIP

中国农业科学 2011,44(19):4022-4028 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.19.012 茉莉酸合成相关基因 Spr2 与 LePrs 在番茄抗根结线虫中的作用 张立宁，程继鸿，杨 瑞，孙中华，吴春霞，王绍辉 （北京农学院植物科学技术学院/农业应用新技术北京市重点实验室，北京 102206） 摘要：【目的】研究茉莉酸合成相关基因Spr2与LePrs对番茄抗根结线虫的影响，探讨茉莉酸在番茄抗根结 线虫病中的作用。【方法】以番茄茉莉酸合成突变体spr2、茉莉酸过量表达转基因番茄 35S::PS及野生型CM为试 材，研究外源喷施茉莉酸甲酯及不同番茄试材相互嫁接对接种根结线虫后番茄根结数、蛋白质酶抑制剂 PI-II 表 达及 Spr2、LePrs 基因转录水平的影响。【结果】番茄茉莉酸合成突变体spr2 较野生型与茉莉酸过量表达转基因 番茄 35S::PS 容易感染根结线虫，外源喷施茉莉酸甲酯降低了番茄根结数。以茉莉酸过量表达转基因番茄 35S::PS 为砧木，可以提高嫁接植株对根结线虫的抵抗能力。【结论】Spr2 突变降低了番茄对根结线虫的抗性， LePrs 过 量表达可以提高植株对根结线虫的抗性。因此，茉莉酸在番茄抗根结线虫病中起到了一定的作用。 关键词：番茄茉莉酸突变体；南方根结线虫；PI-II蛋白酶抑制剂；Spr2；LePrs Effects of JA Synthesis-Related Genes Spr2 and LePrs on the Resistance to Root-Knot Nematodes in Tomato ZHANG Li-ning, CHENG Ji-hong, YANG Rui, SUN Zhong-hua, WU Chun-xia, WANG Shao-hui (Plant Science and Technology College, Beijing University of Agriculture/New Technological Laboratory in Agriculture Application in Beijing, Beijing 102206) Abstract: 【Objective 】 Spr2 and LePrs were related with JA synthesis, the effects of the two genes on the response of tomato to root-knot nematodes were studied. 【Method 】 In this paper, JA biosynthetic mutant (spr2 plants) and JA-overexpression transgenic plants (35S::PS plants) of tomato as well as wild tomato species (CM) were used, and the effects of MeJA sprays and grafting on root-knot numbers, PI-II content and Spr2, LePrs transcription levels, before and after inoculating root-knot nematodes, were analyzed. 【Result 】 Compared with CM and 35S::PS plants, spr2 plants was more susceptible to root-knot nematodes. Spraying MeJA on tomato leaves could