香菇担孢子交配型比例偏分离的遗传分析.pdfVIP

中国农业科学 2007,40(10):2296-2302 Scientia Agricultura Sinica 香菇担孢子交配型比例偏分离的遗传分析 1，2 2 程水明 ，林范学 1 2 （黄冈师范学院生命科学与工程学院，黄冈 438000 ； 华中农业大学农业微生物学国家重点实验室/应用真菌研究所，武汉 430070 ） 摘要：【目的】验证香菇中担孢子交配型因子分离是否存在普遍意义的偏分离现象及其在栽培菌株与野生菌 株中的表现。【方法】以 17 个野生和栽培香菇菌株为样本，采用交配型分析、OWE-SOJ 技术鉴定孢子的交配型， 对各类交配型的比例进行统计分析。【结果】占供试总数 64.71%的菌株担孢子交配型不呈预期的分离比，其中 12 个供试栽培菌株中有 9 个，5 个供试野生菌株中有 2 个；偏分离菌株中均有亲本型孢子数量多于重组型孢子的趋 势，偏分离双核菌丝体的F1 代担孢子的核型主要取决于双核亲本的组成。【结论】香菇中担孢子交配型因子分离偏 离理论预期是一种统计学意义的普遍现象；偏离程度栽培菌株大于野生菌株。 关键词：交配型因子；担孢子；香菇；偏分离 Genetic Analysis of Distorted Segregation Ratio of Mating Types Among Basidiospores in Lentinula edodes CHENG Shui-ming1, 2, LIN Fan-xue2 (1 College of Life Science and Engineering, Huanggang Normal University, Huanggang 438000; 2 State Key Laboratory of Agricultural Microbiology, Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070 ) Abstract: 【Objective 】The objectives of this study are to proof whether or not it exhibits commonly statistical meanings of the phenomenon of mating-type factors segregation in Lentinula edodes spores and to explore the degree of distortion in all tested wild strains and cultivated strains. 【Method 】Seventeen strains of Lentinula edodes, including wild and cultivated strains were used as test materials, statistical analysis on the ratios of spores from different aspects by using mating types analysis and OWE-SOJ technique. 【Result 】Eleven out of the seventeen samples, up to 64.71% of all tested strains, did not display the expected segregation ratio of badidiospores; the nuclear type of F1 progeny spores mainly depended on combined state of parental dikary