小麦籽粒蛋白质含量的动态QTL定位.pdfVIP

中国农业科学 2011,44(15):3078-3085 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.15.002 小麦籽粒蛋白质含量的动态QTL 定位 朱占玲，刘 宾，田 宾，谢全刚，李文福，田纪春 （山东农业大学农学院/作物生物学国家重点实验室小麦品质育种研究室，山东泰安 271018 ） 摘要：【目的】检测灌浆过程中控制小麦籽粒蛋白质含量（GPC）的条件及非条件 QTL，阐明不同时期及不同 时段内QTL 的表达方式，揭示籽粒蛋白质积累的分子遗传机理。【方法】以花培3 号×豫麦57 的168 个双单倍体 （doubled haploid，DH）群体为材料，于6 个不同的环境下种植，在籽粒灌浆的5 个时期取样，对小麦GPC 进行 动态QTL 分析。【结果】共检测到影响GPC 的9 个非条件QTL 和 10 个条件QTL。QGpc3A 为整个灌浆过程都能表达 的非条件QTL，其余条件和非条件QTL 只在几个或单独一个时期表达。花后12 d，控制GPC 的基因表达活跃，非 条件QTL 和条件QTL 总共能解释表型变异贡献率的42.62%；花后22 d，条件QTL 和非条件QTL 总共可解释表型变 异的贡献率较低，仅为 17.43%，GPC 降到“低谷”。 QGpc4A-1 对GPC 前期积累有重要意义，QGpc1D 和QGpc4A-2 对GPC 灌浆中后期积累有重要意义。【结论】GPC 呈现出“高-低-高”的变化规律，控制GPC 的基因在灌浆过程中 以一定的时空方式表达。 关键词：普通小麦；DH 群体；籽粒蛋白质含量（GPC）；非条件QTL；条件QTL Dynamic QTL Mapping of Wheat Protein Content in Developing Grains ZHU Zhan-ling, LIU Bin, TIAN Bin, XIE Quan-gang, LI Wen-fu, TIAN Ji-chun (State Key Laboratory of Crop Biology, Group of Quality Wheat Breeding/ College of Agronomy, Shandong Agricultural University, Taian 271018, Shandong) Abstract: 【Objective 】Grain protein content (GPC) is an important quality factor in bread wheat, which is determined by quantitative trait loci (QTLs). In this study, to attain more genetic information of GPC accumulation，the number and genetic effects of QTLs/genes related to GPC were detected at different seed filling stages. 【Method 】QTLs/genes related to GPC were studied at five seed filling stages in six different environments, using a doubled haploid (DH) population derived from a cross between two elite Chinese wheat cultivars Huapei 3×Yumai 57. QTL mapping for developmental behavior of GPC was studied by unconditional and con