The Effect of Chemical Cues on the Settlement of Sea Cucumber (Apostichopusjaponicus) Larvae.pdfVIP
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J.OceanUniv.China (OceanicandCoastalSeaResearch)
DOI10.1007/sl1802.014.2136.1
ISSN1672-5182,201413(2):321·330
tp://www.ouc.edu.cn/xbywb/
E—mail:xbywb@ouc.edu.cn
TheE仃ect0fChemicalCuesontheSettlementofSea
CucumberA(postichopusjaponicus)Larvae
SUNXiujun,LIQi,YUHong,andKONGLingfeng
KeyLaboratoryofMaricultureofMinistryofEducationofChina,OceanUniversRyofChina,Qingdao266003,PR.China
(ReceivedAugust15,2012;revisedSeptember30,2012:acceptedJuly17,2013)
@OceanUniversityofChina.SciencePressandSpring-VerlagBerlinHeidelberg2014
Abstract TheeffectsoffourionsandeightneuroactivecompoundsoninducinglarvalsealementofA./aponicuswereassessedin
tl1epresentstudy.Al】bioassayswereconductedin60x9nqm Petridisbes.eachcontained10mLofthetestsolutionna d10doliolaria
larvae.Thereweresignificantinductivee ctsof (10mmolL-),NH4 (0.1mmolL ),GABA (1 ’molL_。),acetylcholine(10
molL一),L.DOPA f10一molL一1,norepinephrine(10一molL~1naddopamine(10一molL一nad10‘molL一)‘onthesettlementof
seacucumberlarvae.L.DOPAanddopaminearehtemoste衢cientchemica1cuestoinduceA./aponicus1arvaetoscale.Thehighest
percentageoflarvalsettlementwasinducedby10’molL~L.DOPAnaddopamine(33%nad40%1comparedtothecontrol(7%1.
Howeve~Caz*,Mg ,choline。serotonin,andepinephrinewerelesseffectiveonlarvalsettlementatalltestedconcenrtations.This
sutdyevaluatedthestabilityandfeasibiliyt ofchemicalcuesforlarvalsettlementindifferentculutresystems.whichcanbeapplied
toimprovehtehatcheryproductionofthisvaluablespecies.
Keywords Apostichopusjaponieus;seacucumber;chemicalcue;settlement;larva
PinctadafucatamartensiiandP.maxima(Zhaoeta1.。
1IntroduCtiOn 2003;Yu a1..2008).TheobviouseffectofNH4C1(1ess
than 10mmolL 、onsettlementwasobservedinhtelar-
Larva1settlementandmetamorphosisarecriticaleven~ vaeofoysterCrassostreagigQs(Cooneta1.,1990)nad
in thelife cycleofmostbenhticmar
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