中国马铃薯疮痂病菌的鉴定.pdfVIP

中国农业科学 2006,39(2):313-318 Scientia Agricultura Sinica 中国马铃薯疮痂病菌的鉴定 赵伟全，杨文香，李亚宁，刘大群，孟庆芳，张 汀 （河北农业大学植物病理系，河北省农作物病虫害生物防治工程技术研究中心 保定 071001 ） 摘要: 【目的】对采自中国 10 个省（区）的马铃薯疮痂病菌进行分子水平鉴定。【方法】采用结合生物学特 性和 16S rDNA 序列特点的方法对获得的菌株进行分析。【结果】中国的Streptomyces scabies 菌株与美国的 S. scabies标准菌株ATCC 49173的16S rDNA序列同源性为100%，但中国的菌株不能以甘露醇为单一碳源，对10 IU·ml-1 青霉素不敏感，能产生硫化氢。而中国的 S. acidiscabies 菌株与美国的 S. acidiscabies 标准菌株 ATCC 49003 的 16S rDNA 序列同源性为 99.8%，但能以棉子糖为单一碳源，对苯酚（0.1%）和结晶紫（0.5 µg·ml-1 ）敏感，不 产生硫化氢。另有一种病原链霉菌与所知的链霉菌种均不能归为一类，可能为一个新种。【结论】研究结果表明造 成中国马铃薯疮痂病的病原菌至少有3种，分别为 S. scabies、S. acidiscabies 和一个新种。 关键词：马铃薯疮痂病；链霉菌；生物学特性；16S rDNA序列；鉴定 Characterization and Identification on the Pathogen of Potato Scab in China ZHAO Wei-quan ，YANG Wen-xiang ，LI Ya-ning ，LIU Da-qun ，MENG Qing-fang ，ZHANG Ting (Department of Plant Pathology ，Agricultural University of Hebei ，Biological Control Center of Plant Diseases and Plant Pests of Hebei Province ，Baoding 071001) Abstract: 【Objective 】The pathogens causing potato scab in ten provinces in China were identified. 【Method 】 The scab-related strains were analyzed according to the biological characteristics and the 16S rDNA sequences. 【Result 】The Streptomyces scabies strains from China have the same 16S rDNA sequences to standard S. scabies strain ATCC 49173 from America. But their biological characteristics are different. Chinese Streptomyces scabies strains can not use D-mannitol as single carbon sources, unsensitive to penicillin G (10 IU·ml-1) and produce the H S. The 16S rDNA sequences of Chinese S. acidiscabies strains are 2 similar to standard S. acidiscabies one ATCC 49003(99.8%). It can