乳腺浸润性导管中sparc、kiss-1和kai1的表达和意义.pdfVIP

乳腺浸润性导管癌中SPARC、KISS-1 和KAI1 的表达和意义 摘 要 目的：探讨富含半胱氨酸的酸性分泌蛋白(SPARC)、KISS-1及KAI1在乳 腺浸润性导管癌中的表达和意义及相互之间关系。方法：运用免疫组化 En Vision二步法检测61例乳腺浸润性导管癌、30例伴有癌转移的淋巴结 及32例对照乳腺组织中SPARC、KISS-1及KAI1的表达情况。结果：SPARC 在乳腺浸润性导管癌的癌细胞及间质细胞均有表达。SPARC在淋巴结转 移组中癌细胞的阳性率（47 ％，14/30）高于淋巴结未转移组（23% ，7/31 ） （p0.05 ）；在孕激素受体阳性组癌细胞阳性率（20% ，6/30）低于孕激 素受体阴性组（48% ，15/31）（p0.05 ）。SPARC在乳腺癌间质细胞阳性 表达率70 ％（43/61 ），显著高于对照组（0/32 ）（p=0.000 ）。肿瘤上皮及 间质细胞的SPARC表达与患者年龄、肿瘤大小、组织学分级、TNM分期 及ER 、CerbB-2的表达均不相关（p0.05 ）。转移阳性的淋巴结中的癌细 胞SPARC的表达与原发部位肿瘤分期相关（p0.05 ）。KISS-1蛋白和KAI1 蛋白在乳腺浸润性导管癌中的表达均显著低于对照组（p0.05 ），且在淋 巴结转移组中的阳性率均显著低于淋巴结未转移组（p0.05 ）。乳腺浸润 性导管癌中SPARC蛋白和KISS-1蛋白表达呈负相关（r = －0.335， P0.01 ）。结论：SPARC与乳腺浸润性导管癌的发生发展和转移密切相关。 １ EXPRESSION AND SIGNIFICANCE OF SPARC, KISS-1 AND KAI1 IN INVASIVE DUCTAL CARCINOMA OF BREAST ABSTRACT Objective : To investigate the expression and significance of secreted protein acidic and rich in cysteine (SPARC), KISS-1 and KAI1 in invasive ductal carcinomas of the breast and their relationship. Method: SPARC, KISS-1 and KAI1 protein was detected by immunohistochemistry (En Vision method) in 61 case of invasive ductal carcinoma of the breast, 30 cases of lymph node with carcinoma metastasis and 32 cases of control breast tissue. Results : SPARC protein was expressed in both tumor cells and stromal cells of the breast carcinoma. Expression of SPARC in tumor cells was related to lymph node metastasis (p0.05), and showed lower in the group of patients with progesterone receptor (PR) positive (20%) than that with PR negative (48%)(p0.05). 70 ％（43/61 ）cases of tumor group showed stromal cells positive of SPARC and negative in the control group with significant difference （p0.01 ）.There were no significant associations between ３ expression of SPARC in tumor cells and stromal cells and any of the following factors :