a self-referencing biosensor for real-time monitoring of physiological atp transport in plant systems：一种植物系统中生理生化转运实时监测的自参考生物传感器.pdfVIP

Biosensors and Bioelectronics 74 (2015) 37–44 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios A self-referencing biosensor for real-time monitoring of physiological ATP transport in plant systems Diana C. Vanegas a,b, Greg Clark c, Ashley E. Cannon c, Stanley Roux c, Prachee Chaturvedi d, Eric S. McLamore a,n a Agricultural and Biological Engineering Department, University of Florida, Gainesville, USA b Food Engineering Department, Universidad del Valle, Cali, Colombia c Department of Molecular Biosciences, University of Texas, Austin, USA d Department of Mechanical Engineering, University of Colorado, Denver, USA a r t i c l e i n f o a b s t r a c t Article history: The objective of this study was to develop a self-referencing electrochemical biosensor for the direct Received 1 April 2015 measurement of ATP flux into the extracellular matrix by living cells/organisms. The working mechanism Received in revised form of the developed biosensor is based on the activity of glycerol kinase and glycerol-3-phosphate oxidase. A 9 May 2015 stratified bi-enzyme nanocomposite was created using a protein-templated silica sol gel encapsulation Accepted 9 May 2015 technique on top of graphene-modified platinum electrodes. The biosensor exhibited excellent electro- Available online 29 May 2015 chemical performance with a sensitivity of 2.4 71.8 nA/mM, a response ti