卵巢癌患者腹水xosome的来源及临床意义探讨.pdf

中文摘要 目的证实卵巢癌患者腹水中存在Exosome，探讨其来源及临床意义。 方法(1)通过差速一蔗糖密度梯度离心从41名晚期上皮性卵巢癌患 者的腹水中分离Exosome，用负染电镜技术和Western Blot技术进行 Exosome鉴定即形态观察和分子标志检测；(2)用Westernblot和胶 的来源；(3)分析腹水患者的临床特点，探讨Exosome存在的临床 意义。 结果85．4％(35／41)晚期上皮性卵巢癌患者的腹水中存在直径 术后CAl25平均下降幅度大于患者总体平均降幅(P0．01)。 结论大多数晚期上皮性卵巢癌患者腹水中存在多细胞来源的 后的关系有待进一步研究。 关键词Exosome；卵巢癌；Westernblot；免疫电镜；组蛋白；预后 中图分类号R737．31 Abstract To the ofascites-derivedexosomesfrom objectiveinvestigateorigin withovarian the patients epithelialcarcinoma，and explore betweenexosomesand ofovarian relationship prognosis epithelial carcinoma． MethodsAscites-derivedexosomeswereisolated (1) by onsucroseand from41ovarian D20 ultracentrifugation gradients carcinoma identifiedtransmissionelectron epithelial patients，andby and Westernblot exosomes microscope analyses；(2)Ascites—derived wereexaminedWesternblot andimmunoelectron by analyses for microscopyantigensexpression；(3)UsingstatiStics， ascites-derivedexosomeswereevaluatedforeffecton of prognosis ovarian carcinoma． epithelial ResultExosomeswiththesizeof50—150nmwereisolatedand purified fromtheascitesin ovarian carcinoma 85．4％(35／41)ofepithelial