正常木与应压木木材形成组织中差异表达蛋白质的鉴定.pdfVIP

第35卷摇 第3期 北 京 林 业 大 学 学 报 Vol.35,No.3 2013年5月 JOURNAL OF BEIJING FORESTRY UNIVERSITY May,2013 正常木与应压木木材形成组织中差异表达蛋白质的鉴定 1,2 2 3 2 石江涛 摇 刘一星摇 聂玉哲摇 李摇 坚 1南京林业大学木材工业学院摇 2 东北林业大学材料科学与工程学院摇 3 东北林业大学大庆生物技术研究院 摘要:为了鉴定应压木木材细胞壁形成中的差异表达蛋白质,并确定其在木材形成中的功能,采用双向差异凝胶电 泳技术,对正常木和应压木木材形成组织中蛋白质进行分离和鉴定,获得了较高质量的双向凝胶电泳图谱。 应用 TOF鄄MS/ MS技术对应压木和正常木木材形成组织中的差异表达蛋白质进行鉴定,并用 MASCOT 软件对每个差异 点的匹配峰值在Swiss Prot数据库中进行检索,最终确定了7个差异蛋白质。 其中,4个蛋白质在应压木中表达上 调,3个表达下调。 这些蛋白质参与了木质素生物合成、应力响应与胁迫、细胞骨架运动及脂类代谢,因而推测它们 与应压木木材的形成有关。 关键词:应压木;木材形成;差异蛋白质 鄄鄄 鄄鄄 鄄鄄 中图分类号:S781郾1摇 摇 文献标志码:A摇 摇 文章编号:1000 1522 2013 03 0137 06 1,2 2 3 2 SHI Jiang鄄tao ;LIU Yi鄄xing ;NIE Yu鄄zhe ;LI Jian . Identification of differential protein in the wood forming tissue of normal wood and reaction wood. Journal of Beijing Forestry University 鄄鄄 2013 35 3 137 142 [Ch,30 ref.] 1College of Wood Science and Technology,Nanjing Forestry University,210037,P.R. China; 2 College of Material Science and Engineering,Northeast Forestry University,Harbin,150040,P. R. China; 3 Daqing Institute of Biotechnology,Northeast Forestry University,Heilongjiang,163311,P. R. China. In order to identify the differential proteins in compression wood cell wall formation and determine theirfunctioninwoodformation,two鄄dimensiondifferencein鄄gelelectrophoresiswasemployedtoseparate and identify differential proteins in wood forming tissue of normal and compression wood. The gel electrophoresis map was higher performance. The differential expressed protein was analyzed and found that the changing fold of seventeen spotswas more than twice. Mass identification was carried out using TOF鄄MS/ MSandthepeptidefragmentsweresearchedinSwissProtdatabaseby MASCOT software. Seven proteinswere confirmed,four were up regulated and three were down regulated in compression wood. These proteins were involved in lignin biosynthesis, stress response, cytoskeleton motion and lipid metabolism. Presumably,they were connected with the formation of compression wood. Key words摇 compression wood;wood f