六神丸联合化疗疗中晚期胃癌的临床与诱导肿瘤细胞凋亡的实验研究.pdfVIP

Clinical Study of “Liu-shen-wan ”Combined with Chemotherapy in Treating Advanced Gastric Cancer and Experimental Research in Inducing Apoptosis of Tumor Cell Speciality: Internal Medicine of TCM Author: Wang Xiujuan Tutor: Professor Li Xiurong Abstract Objective: The study observes “Liu-shen-wan ”combined with chemotherapy curative effect to patients of advanced gastric cancer and the mechanism action of “Liu-shen-wan ” inducing the apoptosis of human lung adenocarcinoma cell line A549.Methods: In the part of clinical study, 43 cases with advanced gastric cancers were randomly divided into two groups, 22 patients in the treated group were administered with “Liu-shen-wan ”and chemotherapy (OXA+5-Fu+LV regimen), 21 in the control group with chemotherapy alone. Objective effects and the changes of clinical symptoms were studied after two periods of treatment. In part of vitro cell experiment, mainly use immunohistochemical staining,flow cytometric and RT-PCR technology to observe the mechanism of “Liu-shen-wan ”inducing the apoptosis of human lung adenocarcinoma cell line A549. Results: Clinical research confirmed: tumor patients’ clinical symptoms and quality of life were significantly improved in the treated group, better than in the control one. But there was no obvious difference between two groups on the size of focus and toxic and side-effect; Experimental study confirmed: “Liu-shen-wan ”can induce the apoptosis of human lung adenocarcinoma cell line A549 by raising the level of Caspase-3 and reducing the level of Bcl-2 and Survivin, and combined with DDP can generate synergy. Conclusion: “Liu-shen-wan ”can significantly improve tumor patients’ clinical symptoms and enhance the quality of tumor patients’ life; “Liu-shen-wan ”can play antitumor