寒痹康汤对实验关节炎大鼠血清IL-1β及IL-4的影响.pdfVIP

目 录 中文摘要 ················································1 英文摘要 ················································3 引 言 ···············································5 正 文 ················································8 1.实验材料与方法 ······································8 1.1 实验对象及实验分组 ·································8 1.2 实验药物 ···········································8 1.3 实验方法 ···········································8 1.4 检测指标及方法 ··································9 1.5 统计学方法 ······································11 2.结果和分析 ··········································11 2.1 动物观察 ···········································11 2.2关节肿胀指数观察 ·····························12 2.3 血清 IL- 1 β、IL-4 测定 ···························13 3.讨论·················································13 4.存在的问题与展望 ·································35 结 论 ···············································37 参考文献 ··············································38 英文缩略词表 ··········································45 综 述 ···············································46 致 谢 ···············································56 攻读学位期间发表的学术论文 目录 ····················57 作者声明 ··············································58 个人简历 ··············································59 中 文 摘 要 目 的： 通过本课题的研究观察寒痹康汤对实验性关节炎大鼠血清白介素－ 1 β(Interleukin - 1β, IL-1β)及白介素－4（Interleukin - 4, IL-4 ）的影响， 初步探讨寒痹康汤治疗类风湿关节炎（Rheumatiod Arthritis ，RA ）的作用 机制，并为临床上运用寒痹康汤防治类风湿关节炎提供实验依据。 方 法： 将60只SD雌性大鼠随机分为6组(模型组、甲氨蝶呤组、寒痹康汤高 剂量组、寒痹康汤中剂量组、寒痹康汤低剂量组、正常组)。除正常组外， 模型组、甲氨蝶呤组、寒痹康汤高剂量组、寒痹康汤中剂量组、寒痹康 汤低剂量组每只大鼠颈部、背部皮下多点注射牛II型胶原蛋白与弗氏完全 佐剂混液0.2ml造模，造模前测量所有大鼠足趾部厚度（mm ）。正常组自 由饮水，模型组生理盐水灌胃2ml/ （100g 〃d ），甲氨蝶呤组和寒痹康汤 组初次免疫14天后开始灌胃，甲氨蝶呤组予甲氨蝶呤0.5mg/ (100g 〃w)， 寒痹康汤组分别予寒痹康汤7g/ (100g 〃d) 、3.5g/ (100g 〃d) 、 1.75g/ (100g 〃d)。连续给药28天，于末次给药24h后，测量所有大鼠足 趾部厚度，然后将所有大