半胱氨酸荧光分子探针的研究进展教程分析.doc

半胱氨酸荧光分子探针的研究进展 The research progress of fluorescent molecular probes for cysteine 摘要：近些年来，分子探针因其响应速度快、灵敏度高、使用方便、检测限低等优点应用于对多种分析物的检测。半胱氨酸在很多生理过程中有着重要的作用，因此科研者们制备了多种荧光分子探针来检测半胱氨酸。这篇综述分类总结了基于不同响应机理的半胱氨酸荧光分子探针的研究进展。 Abstract In recent years, while molecular probes have advantages of instantaneous response, high sensitivity, convenient operation and low detection limit, a large number of molecular probes have been developed for various analytes. Scientific researchers have developed a series of fluorescent molecular probes for cysteine homocysteine (Hcy)，because they play crucial roles in many physiological processes. This tutorial review will classify these probes based on different response mechanism. 引言 氨基酸是构成蛋白质的基本物质，并且与生物的生命活动有着密切的联系[1,2]。半胱氨酸(Cysteine，Cys)和同型半胱氨酸(Homocysteine，Hey)是含有巯基（-SH）并且结构相差很小的两种氨基酸，在生理过程中有着重要的作用，医学研究表明他们与很多疾病有关，比如肾功能衰竭老年痴呆症帕金森疾病心血管疾病、冠心病因此，电化学检测光学分析和质谱鉴定这些方法在体外监测，活细胞中的。已经化学反应探针，例如Michael加成醛环化反应裂解反应。在这些方法中，子内荧光淬灭剂2,4-二硝基-苯磺酸盐或2,4-二硝基苯磺酰胺基团诱导的裂解， Kemp M, Go Y M, Jones D P. et al. Nonequilibrium thermodynamics of thiol/disulfide redox systems: A perspective on redox systems biology[J]. Free Radical Biol. Med., 2008, 44: 921–937. [2] Ball R O, Courtney-Martin G, Pencharz P B. The in vivo sparing of methionine by cysteine in sulfur amino acid requirements in animal models and adult humans[J]. J. Nutr., 2006, 136: 1682S–1693S. [3] Giles N M, Watts A B, Giles G I, et al. Metal and redox modulation of cysteine protein function[J]. Chem. Biol., 2003, 10: 677–693. [4] Wang W, Rusin O, Escobedo J O, et al. Detection of Homocysteine and Cysteine[J]. J. Am. Chem. Soc., 2005, 127, 15949–15958. [5] Seshadri S, Beiser A, Selhub J, et al. Plasma homocysteine as a risk factor for dementia and Alzheimers disease[J]. N. Engl. J. Med., 2002, 346: 476–483. [6] Obeid R, Herrmann W. Mechanisms of homocysteine neurotoxicity in neurodegenerative diseases with special reference to dementia[J]. FEBS Lett., 2006, 580: 2994–3005. [7] Pastore A, Federici G, Bertini E,