基于纳米金和硫堇固定酶的过氧化氢生物传感器答辩人：陈资料.ppt

 基于纳米金和硫堇固定酶 的过氧化氢生物传感器 答辩人：陈贤光（03应化） 指导老师：童叶翔 教授 邹小勇 教授2006.6 广州 Hydrogen Peroxide Biosensor Based on Immobilizing Enzyme by Gold Nanoparticles and Thionine① CHEN, Xian-Guang ZOU, Xiao-Yong TONG, Ye-Xiang*School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China ① This paper will submit to Acta Chim. Sinica . 1. Introduction1.1 Mechanism of Hydrogen Peroxide Biosensor Guilbault G. G. , et. al. Anal. Chim. Acta. , 1973, 64: 439-445. 1. Introduction 1.2 Fabrication of hydrogen peroxide biosensor made in our work 1. Introduction 1.3 Catalytic reaction of H2O2 appear in the system 2. Results and Discussion 2.1 Prepare GNs by sol-gel method Fig. 1 TEM image of GNs. ( d = 20 nm ) 2. Results and Discussion 2.2 Electrochemical impedance spectrum (EIS) Fig. 2 Nyquist plot of EIS of the electrodes (a) and equivalent circuit (b) of the biosensor. (a) (b) Rapp : electrode apparent resistance; Rsol : solution resistance; Rct : charge transfer resistance. Electrode Rapp / Ω Rsol / Ω Rct / Ω a 1179 9326 1724 b 2208 9623 6334 c 3536 9882 3780 d 5016 144.6 7580 Table 1 Electrochemistry parameters 2. Results and Discussion 2.3 Atomic force microscopy (AFM) images Fig. 3 Three-dimensional AFM images of bare Pt (a), Pt/GNs (b), Pt/GNs/Thio (c) and Pt/GNs/Thio/(HRP-GNs) (d) electrode. 2. Results and Discussion 2.4 Cyclic voltammgrams (CVs) (b) Fig. 5 CVs of electrocatalysis of the biosensor to H2O2 in pH 7.0 PBS containing 1×10-3 mol/L hydroquinone. (a) Fig. 4 CVs (a) of the biosensor at different scan rates in blank pH 7.0 PBS and the linear calibration curve between scan rates and cathode peak currents (b). 2. Results and Discussion 2.5 Some factors effect on the response currents Fig. 6 Effect of applied potential on the response currents of the biosensor to 2×10-4 mol/L H2O2. Other conditions