甘肃现代肉羊新品种群羊GH基因外显子1多态性及体重关联分析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 12 GH 1 张浩 朱爱文 马友记 李发弟 陈国宏 (, 730070) : 根据 GenBank发表的绵羊生长激素( GH )基因外显子 1的序列 计 一对引物, 采用 PCRSSCP技术分析 GH 基 因外显子 1在甘肃现代肉羊新品种选育群羊中的单核苷酸多态性, 并与 3月龄前的体重进行关联分析结果表明, GH 基因 外显子 1在新品种群羊中存在多态性, 检测到两种基因型(AAAB), 其 301 bp处有 一个T /A 突变和 305 bp处有 一个G /A 突 变, 初生重1月2月3月龄体重 AAAB型都无显著性差异 (P 005), 但 3月龄 AB 型 个体的体重相对于 AA 型偏高, 由此 初步推断 GH 基因可能是影响绵羊体重性状的主基因或与主基因相连锁, 可用以对绵羊体重性状进行标记辅助选择 : 生长激素基因 绵羊 PCRSSCP 遗传多态性 体重 Polymorphism of Exon 1 of GrowthHormoneGene and ItsRelationship with BodyWeight of the GansuModernMeat Sheep New Breed Population Zhang H ao Zh A wi en M aYo ji Li Fadi Chen G ohong (College of A nimal Science and Techno logy, GansuAgricultural University, Lanzhou 730070) Abstrac:t A pair of prmi erswas designed according to the seq ence of exon 1 of grow th horm one ( GH ) gene p blished in Gen Bank. S ingle n cleotide polymorph ism ( SNPs) of exon 1 of GH genew as detected in Gans m odern meat sheep new breed pop lation by polym erase chain reaction ( PCR ) single strand conform ation polymorph ism ( SSCP) . The res lts showed that the polym orph ism w as detected and had wt o genotype(AA, AB) in Gans modern m eat sheep new breed pop lation, wh ich have aT /A m tation in 301 bp and G /A m tation in 305 bp of exon 1 of GH Gene. The correlation between these two genotypes w ith body w eight w as analyzed, show ing that the body w eigh t of b irth, onem onth, two m on th and threem onth w ere not sign ifican t w ith different genotypes(P 005), in add i tion, AB are h igher than AA. W e concl ded that GH gene possibly is m ajor gene or linked to am ajor gene that effect the sheep body w eight traits, and carries on them arkerassisted selection to bo