棉花高频体细胞胚胎发生及其再生体系建立.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 20 10 8 顾冉冉 薛金教 袁英歌 彭明 崔百明 ( , 83200 ) : 棉花的体细胞胚胎发生率低, 限制了转基因棉花的发展 了扩大可再生棉花的基因型, 发展新疆优质棉花 的特点, 以新疆的主要栽培品种新陆早 33 材料, 以下胚轴 外植体, 使用葡萄糖, 麦芽糖作 主要碳源, 用 Phytage l固化, 对 不同激素的浓度组合和培养条件进行探索, 对棉花胚性愈伤的诱导到体细胞胚胎的发生阶段进行优化, 体细胞胚胎成熟以后 进行萌发培养可以获得正常的植株通过试验证明, 有利于棉 花的愈伤组织生长的激素浓度 KT 01 mg /L, 2, 4D 01m g/L, 下胚轴的中部诱导愈伤形态最好体细胞胚胎发生阶段以 KT, IBA 组合, 以低盐培养基进行子叶胚的成苗, 建立了再生体系 : 棉花 体细胞胚胎 再生植株 组织培养 H igh Efficiency of Somatic Embryogenesis and Plant Regeneration from Cotton(Gossyp ium h irsutum L. ) Gu Ran an X ue Jinjiao Y uan Y ingge Peng M ing Cu i Ba mi ing (Key Laboratory of Agricultureand B iotechnology, College of Lfie Sciences, Shihezi University, Shihezi 832000) Abstrac:t Low effic iency of som atic emb yogenesis of Cotton, wh ich lmi its the developm ent of genetically mod ified co tton. In o de to expand enew ab le co tton geno types and dev elop the cha acte istics of h ighquality cotton in X inj iang, the m a in cultiv a of X in j iang X inluzao 33 fo m ate ia ls, the fo llow ing axes as exp lants, using g lucose, m altose as the ma in ca bon sou ce, w ith Phy tagel cu e, the concent ation of d iffe ent ho mones to exp lo e the composition and cultu e cond itions fo em b yogen ic ca llus induc tion of co tton to the occu ence of som atic emb yos to optmi ize stag es, som atic em b yos m atu ed fo ge m ina tion of cu ltu ed p lants ge t no ma l. By stud y ing the ev idence in favo of cotton callus g ow th ho m one concen t ation KT 01m g/L, 2, 4D 01mg /L, the cent a l pa t of hypo co tyls ca llus induction the best shape. In the som a tic em b yogenesis stages, KT, IBA po tfo lio togethe w ith lowsa lt med ium fo coty ledon em