蛋白质组学的研究技术及在植物抗渗透胁迫的研究中的应用.pdf

生物技术通报 BI OTECHN OLOG Y B ULLETI N 2005 2 1 1 2 何宝坤 王玉洁 孙立平 1 2 ( , 276005; , 271000) : 蛋白质组学是功能基因组学研究的热点领域之一该文介 了蛋白质组学的基本的和新兴的研究 技术方法如蛋白质组样品的制备双向凝胶电泳生物质谱技术蛋白质芯片技术酵母双杂交系统和生物信息学 等, 以及蛋白质组学技术在植物抗干旱盐渍等渗透胁迫研究中的应用 : 蛋白质组学 研究技术 植物 渗透胁迫 Proteomical Research Technology and its Application in the Research of Plant Resistance to Osmotic Stress 1 1 2 H e Baokun W ang Yujie Sun Liping 1 ( Lin Yi Normal University , linyi 276005; 2 Dep artment of Chemistry and Chemical Engineer ing , Taishan Medical College, Taian 271000) Abstract: Pr oteom ic i one of the mo t active re earch f ield in functional g eno mic . T he article intr oduced the e ential and new protemical re earch technique and method uch a preparat ion of proteome example, two dimen- io nal electrophore i ( 2- DE) , bio-ma pectr ometry ( M S) , protein chip , yea t two hybrid y tem and bio-informat- ic . T heir applicat ion in the r e ear ch of plant re i tance to o mot ic tr e uch a dr ought and alt tre w ere illumi- nated biefly. Key w ords: Pr oteom ic Re ear ch technolo gy Plant O motic tr e / , [ 1] , 1995 1996 [ 2 ] Wilkin William 1994 [ 3] , , , ,