从虎皮鹦鹉粪便中分离产细菌素菌株及细菌素特性分析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 9 陈新练 陈仪本 1 2 ( , 510301; 3 , 510070; , 100049) : 从虎皮鹦鹉(M elop sittacus undula tus)的粪便中分离得到 一株产广谱细菌素的菌株, 其细菌素产物对革兰 阳性 和阴性细菌均有较强抑制作用经形态学生理生化反应和 16S rDNA 分子方法鉴定, 该菌株初步鉴定为干酪乳杆菌用硫 酸铵分级沉淀得到的细菌素粗提物,作温度pH 值蛋白酶的敏感性测定, 结果显示该细菌素经 100 处理 10m in抑菌活性不 变, 当 pH40时, 对大肠杆菌和金黄色葡萄球菌的抑菌率分别达到 9906% 和 8614% , 并且对多种蛋白酶表现出敏感性与 N isin标准品的抑菌效价对比, 得到粗提物的效价为 20487 IU /mL : 细菌素 虎皮鹦鹉 干酪乳杆菌 抑菌试验 抑菌效价 Characteristics of Bacteriocin and BacteriocinproducingBacteria Isolated fromM elop sittacus undulates! Excrement henX inlian hen Y iben 1 2 ( South China Sea Institute of Oceanology, Guangzhou 510301; Guangdong ProvincialK ey La oratory of M icro ialCulture Collection and App lication, Guangdong ProvincialK ey La oratory of Applied M icro iology, Guangdong Institute 3 of M icro iology, Guangzhou 510070; Graduate University of ChineseA cademy of Sciences, Beij ing 100049) Abstrac:t A strain ofbacteria which can produces a broadspectrum bacteriocinw as isolated from the excrement of theM elop sitta cus undu lates. Its product could inhibit not only grampositive bacteria but also gramnegative bacteria efficiently. Detecting its appear ance, physiological and biochem ical characteristics and analyzing 16S rDNA gene sequence homology, the strain of bacteria w as identi fied asLacto acillus casei. The sensitivity of rough bacteriocin to temperature, pH and prote