红麻不育系和保持系中cob基因的克隆及分析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 20 11 2 cob 陈鹏 周瑞阳 赵杨 蒋利和 (, 530004) : 开展红麻的 CMS 机理的研究有利于更好地利用其杂种优势分 提取红麻 CM S系和保持系线粒体 DNA, Sou thern blot分析表明, cob基因的组织形式存在差异根据不同物种 cob基因的保守序列设计引物, 在红麻 CMS系和保持系 中克隆到了 cob基因, GenBank序列号分 为 HM 535 86和 HM 535 8 基因长度均为 1 1 9 bp, 编码 392 个氨基酸残基, 分子 量约为 43 kD序列比较发现 cob基因在 CM S系和保持系同源性达 998% , 和其他物种中 cob基因的同源性大于 923% 研 究结果为下 一步克隆 cob基因的侧翼序列, 进而揭示红麻的 CMS机理提供了很好的研究基础 : 红麻 细胞质雄性不育 线粒体 基因克隆 Cloning and Sequence Analysis of cob Gene in CMS and Its Maintainer Liner ofH ib iscus cannab inus Chen Peng Zhou Ruiyang Zhao Yang Jiang Lihe (College of Agricultura,l Guangxi University,N anning 530004) Abstrac:t E lucidating the m echan ism of CM S of kenaf cou ld be prop itious to m ake good use of its heterosis. m t DNA w ere -i solated from CM S and its m ain tainer liner of kenaf to carry out Sou thern b lot by using cob gene as prob. The resu lt show ed cob gene w as different in organ ization in CM S and m aintainer liner. Fu rtherm ore, cob genew as cloned from CM S and m aintainer liner respectively by a hom ologous m ethod. These tw o genesw ere both 1 1 9 bp in length, encoding a protein of 392 am ino acidsw ith them o lecu larw eigh t about 43 kD. These tw o genes shared 998% identity in nucleotide to each other and over 923% identities to that of other speciesThe resultsw ou ld provide a basis for clon ing the flank sequence of cob gene so as to reveal them echanism of CM S of kenaf. K ey w ords: Kenaf Cytop lasm ic m ale sterility( CM S) M itochondrial Gene clon ing [ 3] (H ibiscus cannabinus L) U gale