穿心莲内酯抑制肝癌细胞生长及对细胞内周期调控相关蛋白的影响（英文）.pdfVIP

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穿心莲内酯抑制肝癌细胞生长及对细胞内周期调控相关蛋白的影响（英文）.pdf

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药学学报 Acta Pharmaceutica Sinica 2009, 44 (9) : 973979 ·973 · Andrographolide inhibits hepatoma cells growth and affects the expression of cell cycle related proteins 1 1 1 1, 2* 1, 2* SHEN Kai-kai , LIU Tian-yu , XU Chong , JI Li-li , WANG Zheng-tao (1. Key Laboratory of Standardization of Chinese M edicines of M inistry of Education, Institute of Chinese Materia M edica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2. Shanghai R D Centre for Standardization of Chinese Medicines, Shanghai 201203, China) Abstract: The present study is aimed to investigate the toxic effects of andrographolide (Andro) on hepatoma cells and elucidate its preliminary mechanisms. After cells were treated with different concentrations of Andro (0 50 μmol·L 1) for 24 h, cell viability was evaluated with 3-(4,5-dimethylthiazol-2-yl) 2,5- diphenyltetrazolium bromide (MTT) assay. Furthermore, after hepatoma cells (Hep3B and HepG2) were treated with different concentrations of Andro (0 30 μmol·L 1) for 14 d, the number of colony formation was accounted under microscope. Cell cycle related proteins such as Cdc-2, phosphorylated-Cdc-2, Cyclin B and Cyclin D 1 were detected with Western blotting assay and the cell cycle was analyzed by flow cytometry using propidium iodide staining. MTT results showed that Andro induced growth inhibition of hepatoma cells in a concentra- tion-dependent manner but had no significant effects on human normal liver L-02 cells. Andro dramatically decreased the colony formation of hepatoma cel