根皮素的抗炎和免疫抑制作用(英文).pdfVIP

480 药学学报 Acta Pharmaceutica Sinica 2009, 44 (5) : 4 80485 Anti-inflammatory and immunosuppressive effect of phloretin * LU Xiao-yu, ZENG Yao-ying , YE Yan-xia, ZHOU Yu-ying, MU Jing-ji ng, ZHAO Xiao-hui (Institute of Tissue Transp lantation and Immunology, Jinan University, Guangzhou 510632, China) Abstract: This study investigated the effect of phloretin (Ph) on the proliferation, activation, and cell-cycle distribution of mouse T lymphocytes and NO production and phagocytosis of macrophages. Carboxyfluo- rescein diacetatesuccinimidyl ester (CFDA-SE) staining plus flow cytometry assay was employed to obtain the proliferation-related index (PI) of lymphocytes. The expression levels of CD69 and CD25 on T lymphocytes stimulated with Con A were evaluated with flow cytometry after staining with fluorescent monoclonal antibody. Cell-cycle distribution of T lymphocytes was analyzed by propidium iodide staining. Griess kit was used to evaluate the NO production and fluorescent microbeads were used to analyze the phagocytosis ability of macro- phages. Our results showed that phloretin (40, 60, and 80 mol·L 1) significantly inhibited the proliferation of T lymphocytes and the PI reduced from 1.4 1 ± 0.13 to 1.34 ± 0.16, 1.19 ± 0.12 and 1.07 ± 0.06, respectively. Phloretin significantly inhibited the expression of CD69 and CD25 (P 0.01). The cell cycle distribution analysis showed that phloretin could induce a cell cycle arrest at G /G phase. NO production of LPS +IFN- 0 1 group of macrophages was (26.72 ± 3.57) mol·L 1, and was significantly reduced by phloretin (P 0.0 1). And phagocytosis rate of macrophages was significantly reduced by ph