蛋白质分子印迹技术研析进展及其应用前景.pdfVIP

132 药学学报 Acta Pharmaceutica Sinica 20 11, 46 (2) : 132 137 蛋白质分子印迹技术的研究进展及应用前景 孙寅静, 罗文卿, 潘 俊* (复旦大学药学院, 上海 201203) 摘要: 分子印迹技术是在聚合物材料的合成过程中构建与模板分子在大小、形状和结构功能上都互补的特 异性结合位点, 这样的材料对其模板具有选择性结合能力。尽管小分子印迹技术近年来发展迅速, 蛋白质分子印 迹却由于蛋白质的体积庞大、结构灵活、构象复杂成为既有意义又具挑战性的研究领域。本文总结了近 年来 蛋白质分子印迹技术的研究报道, 综述了其技术特点、最新进展和应用前景。 关键词: 分子印迹; 蛋白质; 制备; 表征; 应用前景 中图分类号: R943 文献标识码:A 文章编号: 05 13-4870 (20 11) 02-0132-06 Recent advances and perspective in the study of the molecular imprinting of proteins * SUN Yin-j ing, LUO Wen-qing, PAN Jun (School of Pharmacy, Fudan University, Shanghai 201203, China) Abstract: Molecular imprinting technique (MIT) involves the synthesis of polymer in the presence of a template to produce complementary binding sites in terms of its size, shape, and functional group orientation. Such kind of polymer possesses specific recognition ability towards its template molecule. Despite the rapid development of MIT over the years, the maj ority of the template molecules that have been studied are small molecules, while molecular imprinting of proteins remains a significant yet challenging task due to their large size, structural flexibility and complex conformation. In this review, we summarize the research findings over the past five years, and discuss the characteristics of the technique, the most recent progress and the perspective in the field of molecular imprinting of proteins. Key words: molecular imprinting; protein ; pre