第二章蛋白质的定性定量分析.ppt

3. 注意事项 柱床表面不能干燥 凝胶的选择 Sephadex 溶胀 G值的意义 凝胶柱的再生与保存 第 三 节 等电聚焦电泳测定蛋白质的等电点 等电点 (isoelectric point, pI) 两性电解质性质 支持介质 (supporting media) Figure of IEF + – pH 3 pH 7.5 pH 10 + – pH 3 pH 7.5 pH 10 + – pH 3 pH 7.5 pH 10 + – pH 3 pH 7.5 pH 10 Isoelectric Focusing ReadyStripTM IPG Strips 3–10 4–7 3–6 5–8 7–10 3–10NL 3.9–5.1 4.7–5.9 5.5-6.7 6.3-8.3 High-purity monomers Narrow ranges for resolution of low-abundance proteins Overlapping gradients for “cyber gels” Three lengths 7 cm, 11 cm and 17 cm 0.5 mm x 3.3 mm Broad Range pH 3-10 Narrow Range pH 4-7 Micro Range pH 4.7-5.9 3