《Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites》.ppt

Reporter： Chongkun Wang Date：2015.12.10 Email:xldlx_liqun@139.com Literature Reports 东华理工大学化学生物与材料科学学院 Faculty of Biology, Chemistry and Material Science, East China University of Technology Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites (Chongkun Wang. Talanta. 2015) College of Chemistry, Jilin University IF 3.545 Content Background 1 Experimental 2 Results and Discussions 3 Conclusion 4 Background thrombin(TB) detection sensitivity and selectivity electrochemical impedimetric aptasensor EIS Rct essential for diagnosis thrombosis and hemostasis Apparatus and Chemicals Experimental Zahner Zennium electrochemical workstation for EIS CH Instrument 832B electrochemical workstation for CV three electrode electrochemical cell: Ag–AgCl reference electrode,a bare gold working electrode, a platinum wire electrode. JEM-2100F high-resolution transmission electron microscope,HITACHIH-600 Analytical TEM, ESCALAB-MKII spectrometer (VG Co., United Kingdom) Graphene oxide(GO) 、Dithiothreitol(DTT)、Tris(2carbo-xyethyl) phosphine hydrochloride(TCEP)、Rhodamine6G (R6G)、Chlor-oauric acid(HAuCl4 .4H2O)、Tris(hydroxym-ethyl) aminomethane(Tris) Thiolated thrombin aptamers TBA15(5′-HS-SH-(CH2)6-TTT TTT TTG GTT GGT GTG GTT GG-3′) and TBA29(5′-HS-(CH2)6-TTT TTT TTA GTC CGT GGT AGG GCA GGT TGG GGT GAC T) Thrombin,bovine serum albumin(BSA), Trypsin, immunog-lobulinG (IgG) was prepared in 34mM Tris HCl methanol and store at 4℃ rGO–AuNPs: 60mg of sodium citrate was added to homogeneous GO dispersion under refluxing and stirring(2.5h);100μL HAuCl4 was added to the above solution and reflux(30min);rGO–AuNPs dispersion was centrifuged at 12,000 rpm and washed with water and redispersed into 6mL water by sonication. rGO–TBA29: 20μL of TCEPand excess of TBA29 was added to100μL rGO–AuNPs suspension and ediluting to 0.5mL with water in 24h; twice centrifugation at 12,000 rpm for 10min and then add 100μL of 0.1mM R6G. electrode modific