明日叶查尔酮对2型糖尿病大鼠肝细胞胰岛素信号转导分子mRNA表达影响.pdfVIP

The effect of chalcones extracted from Angelica Keiskei on the mRNA expression of insulin signal key molecules in hepatocytes of rats with 够pe 2 diabetes ABSTRACT Objective：To observe the effect of chalcones extracted from Angelica Keiskei(AC)on the mRNA expression of insulin receptor(InsR)、insulin receptor substrate一2(IRS-2)、 Phosphatidy I inositol 3-kinase(P13K)and serine-threonine(Akt)and explore Ashitaba chalcone improvement in type 2 diabetic rats insulin signal transduction disorder． Method：The diabetes of rats was induced by streptozotocin with intraperitoeal injection as well as with high—fat diet feeding．The rats were randomly divided into 3 groups诚th 1 0 rats in each group，diabetic control group，high-dose AC group and low-dose AC group．All the rats were fed、Ⅳith high—fat diet．0．30 and 1 0 mg／kg。BW AC per day were given to high-dose AC，low—dose AC and the diabetic control groups，respectively．Another 1 0 normal rats fed with regular diet Was used as the normal control group．After 4 weeks， serum insulin levels were evaluated by radioimmunoassay．The mRNA expression levels of InsR、IRS一2，P13K and Aktwere determined by reverse transcription polymerase chain reaction(RT-PCR)．The protein expression levels of insulin receptor in hepatocytes were detected by immunohistochemistry．and the phosphorylation levels of Akt in hepmocytes were detected by western blot． Results： (1)the levels of fasting blood glucose and fasting serum insulin：the level of fasting blood glucose in rats ofhigh-dose AC gruop Was 7．0 4-1．12，insulin level Was 29．5 4-5．3l， compared with the diabetic control group，the difference Was statistically significant(P 0。05)． (2)insulin resistance index：the high and low dose groups insulin resistance index were：8．99 4-O．79，23．47 4-5．65，diabetic control group was 28．06 4-3．24，the difference between groups was statistically significant(F=1 3，26，PO．05)．High dose groups were compared with the diabetic control group，the difference was statistically significant(P 0