第十二章抗原提呈细胞对抗原的处理及提呈_分析.pptVIP

抗原的加工处理 一、MHCⅠ类分子途径 （内源性抗原提呈途径） MHCⅠ类分子抗原提呈途径 MHCⅠ类分子抗原提呈途径 1、内源性抗原的加工、处理与转运 1)蛋白酶体,其酶活性组分为低分子量多肽（LMP） 2)抗原加工相关转运体(TAP) 2、MHCⅠ类分子的生成与组装 3、抗原肽-MHCⅠ类分子复合物的形成与抗原肽的提呈 PSMB 8和PSMB9（ 又称LMP-2 和 LMP-7） 编码蛋白酶体β亚单位，具有酶活性。 参与内源性抗原的酶解 蛋白酶体β亚单位基因/LMP基因 （proteasome subunit beta type，PSMB） TAP-1 和 TAP-2 编码内质网膜上的TAP 参与内源性抗原的转运 抗原加工相关转运体基因 （Transporter Associated with antigen Processing,TAP） (胞浆) (内腔) (疏水的) (跨膜的) 抗原加工相关转运体（TAP） MHCⅠ 分子的生成与组装 TAP MHC－I分子的合成 MHC－I，?2m 和抗原肽的组装 运输至膜表面 短肽 内源性蛋白 细 胞 核 CD8+ T 细胞 APC B7 CD28 1 2 3 4 MHCⅠ 类 分 子 抗 原 提 呈 途 径 MHC-I CD8 TCR 抗原肽 蛋白酶体 * The only known function of dendritic cells is to present antigen to naive T cells and activate them. As we know, dendritic cells arise from bone marrow progenitors and migrate via the blood to peripheral tissues and organs. The immature DC in the peripheral tissues are highly phagocytic via receptors such as DEC 205 and are actively macropinocytic but express low levels of MHC proteins, and they lack co-stimulatory B7 molecules. So, they cannot present antigen and they are not yet equipped to stimulate naive T cells. In contrast, the mature DC in lymphoid tissue have high ability to present antigen but are no longer phagocytic. They express B7.1, B7.2, and high levels of MHC class I and class II molecules, as well as high levels of the adhesion molecules ICAM-1, ICAM-2, LFA-1, and LFA-3 (center panel). They also express high levels of the dendritic-cell-specific adhesion molecule DC-SIGN, which binds ICAM-3 with high affinity. * The only known function of dendritic cells is to present antigen to naive T cells and activate them. As we know, dendritic cells arise from bone marrow progenitors and migrate via the blood to peripheral tissues and organs. The immature DC in the peripheral tissues are highly phagocytic via receptors such as DEC 205 and are actively macropinocytic but express low levels of MHC proteins, and they lack co-stimulatory B7 molecules. So, they cannot prese