苦荞EMS诱变群体的创建及农艺性状分析.docVIP

苦荞EMS诱变群体的创建及农艺性状分析 　　摘要：利用化学诱变剂甲基磺酸乙酯 （ethyl methane sulfonate，EMS） 诱变处理苦荞（Fagopyrum tataricum （L.） Gaertn）种子，对M2代突变材料进行生物学性状与农艺性状鉴定和分析，M3代材料进一步验证。结果表明，试验获得了叶片肥大、早熟、晚熟、小粒、矮秆和黄化6种稳定遗传的突变体。叶片肥大、早熟和晚熟突变体的株高、主茎分枝数、主茎节数、株粒数、株粒重和千粒重与对照间均无显著差异。矮秆突变体的株高和主茎分支数以及小粒突变体的株粒重和千粒重显著低于对照，其他性状与对照间差异不显著。黄化突变体的株高与对照无显著差异，主茎分枝数、主茎节数、株粒数、株粒重和千粒重均显著低于对照。 　　关键词：苦荞（Fagopyrum tataricum （L.） Gaertn）；甲基磺酸乙酯；突变体；农艺性状 　　中图分类号：S517 文献标识码：A 文章编号：0439-8114（2015）14-3343-05 　　DOI：10.14088/ki.issn0439-8114.2015.14.004 　　Construction and Agronomic Traits Analysis of Mutant Population from Tartary Buckwheat Induced by Ethyl Methane Sulfonate（EMS） 　　DENG Lin-qiong，ZHANG Yi-zhong 　　（Ecological Engineering School，Guizhou University of Engineering Science， Bijie 551700， Guizhou， China） 　　Abstract： The seeds of tartary buckwheat were treated by chemica1 mutagen ethyl methane sulfonate （EMS） to construct a mutant population. The biological and agronomic characters were identified and analyzed in M2 generation. M3 generation materials were further verified. The results showed that six types of stable genetic mutants（big leaf， premature， late maturity， small grain， dwarf and albino plant） were gotten. There were no significant differences for the plant height， the branches of main stem， the nodes of main stem， the seeds per plant， the weight per plant and the 1000-seeds weight between the big leaf mutant， premature mutant and late maturing mutant. The plant height and the branches of main stem of the dwarf mutant and the weight per plant and the 1000-seeds weight of the small grain mutant were significant lower than the control materials， whereas the other characters were no significant differences with the control materials. Though the plant height of the albino plant was no significant differences with the control materials， the branches of main stem， the nodes of main stem， the seeds per plant， the weight per plant and the 1000-seeds weight were significant lower than the control materials.