Serumpeptidome解析.ppt

Identification and Separation of Proteomics 分离与鉴定蛋白质比核酸困难得 Much more difficult to separate and identify proteins than nucleic acid 对蛋白质组进行表征, 即全部蛋白质的分离、鉴定及图谱制作。 Protocol: First step: separate proteins from samples Second step: identify those proteins Identification and Separation of Proteomics 双向凝胶电泳 two dimentional electrophoresis, 2- DE 蛋白质组多维色谱分离技术 chromatographic fractionation 质谱相关的蛋白质鉴定技术 mass-spectrum 同位素亲和标签技术 isotope- coded affinity tag, ICAT 蛋白质与抗体阵列芯片的检测 protein array Identification and Separation of Proteomics 质谱相关的蛋白质鉴定技术 mass-spectrum 基本原理: 样品分子离子化后, 根据不同离子间质荷比 m