基于网络药理学的罗格列酮复方作用机制探讨_白雨.docxVIP

· 284 · 药学学报 Acta Pharmaceutica Sinica 2015, 50 (3): 284?290 基于网络药理学的罗格列酮复方作用机制探讨 白 雨 1, 2, 范雪梅 2*, 孙 瀚 2, 3, 王义明 1, 2, 梁琼麟 2, 罗国安 1, 2 (1. 江西中医药大学药学院, 江西 南昌 330004; 2. 清华大学化学系生命有机磷化学及 化学生物学教育部重点实验室, 北京 100084; 3. 辽宁中医药大学药学院, 辽宁 大连 116600) 摘要: 在新药研发及药理学研究领域, 网络药理学的应用日渐广泛, 方法层出不穷。本研究以罗格列酮复方 为研究对象, 采用 3 种网络药理学预测方法进行靶点预测和作用机制探讨, 并比较评价 3 种预测方法的异同。研 究发现, 3 种方法预测得到的结果并不相同, 但钙信号转导通路在 3 种方法中均可预测到。经生物信息学分析发 现, 这条通路与糖尿病及糖尿病引起的认知障碍相关, 表明钙信号转导通路是罗格列酮复方作用过程中重要的 信号调节通路。本研究为该复方作用机制的进一步阐释提供了线索, 同时也为实际研究中网络药理学靶点预测 方法的选择和应用提供参考。 关键词: 罗格列酮复方; 网络药理学; 靶点预测; 信号通路; 作用机制 中图分类号: R963 文献标识码: A 文章编号: 0513-4870 (2015) 03-0284-07 The mechanism of rosiglitazone compound based on network pharmacology BAI Yu1, 2, FAN Xue-mei2*, SUN Han2, 3, WANG Yi-ming1, 2, LIANG Qiong-lin2, LUO Guo-an1, 2 (1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China; 2. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China; 3. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China) Abstract: Applications of network pharmacology are increasingly widespread and methods abound in the field of drug development and pharmacological research. In this study, we choose rosiglitazone compound as the object to predict the targets and to discuss the mechanism based on three kinds of prediction methods of network pharmacology. Comparison of the prediction result has identified that the three kinds of prediction methods had their own characteristics: targets and pathways predicted were not in accordance with each other. However, the calcium signaling pathway could be predicted in the three kinds of methods, which associated with diabetes and cognitive impairment caused by diabetes by bioinformatics analysis. The above conclusion indicates that the calcium signaling pathway is important in signal pathway regulation of rosiglitazone compound, wh