StochasticOpticalReconstructionMicroscopy.pptVIP

* Stochastic Optical Reconstruction Microscopy (STORM) 美国哈佛大学年轻的中国学者庄小威教授将荧光光谱和显微分析技术应用于单分子测量，发明了三维随机光重构显微术（3D-STORM）。 Example from Science Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy Bo Huang,1,2 Wenqin Wang,3 Mark Bates,4 Xiaowei Zhuang1,2,3* Science, February 8, 2008 1 Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.2 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.3 Department of Physics, Harvard University, Cambridge, MA 02138, USA.4 School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. Members of team Xiaowei Zhuang, Prof of Chemistry Chemical Biology, Prof of Physics, HHMI, Harvard Bo Huang, post-doctoral fellow in Zhuang lab. Wenqin Wang, graduate student, Dept of Physics, Harvard; member Zhuang lab Mark Bates, graduate student, Division of Engineering and Applied Sciences, Harvard; member of Zhuang lab Finding Out the Position of a Molecule σ ? σPSF / N1/2 Yildiz et al., Science, 2003 Gordon et al., PNAS, 2004 Lagerholm et al., Biophys. J., 2006 FIONA Localization by sequential photobleaching Thompson at al., Biophys. J., 2002 Qu et al., PNAS, 2004 Lydke et al., Optics Exp., 2005 or QD blinking A few molecules * Demonstration of the difference between single- and two-photon excitation The cuvette is filled with a solution of a dye, safranin O, which normally requires green light for excitation. Green light (543 nm) from a continuous-wave helium-neon laser is focused into the cuvette by the lens at upper right. It shows the expected pattern of a continuous cone, brightest near the focus and attenuated to the left. The lens at the lower left focuses an invisible 1046-nm infrared beam from a mode-locked Nd-doped yttrium lanthanum fluoride laser into the cuvette. Because of the two-photon absorption, excitation is confined to a tiny bright spot in the middle of the cuvette. Image source: