硫素水平对韭菜硝酸盐累积和氮代谢关键酶活性的影响.docVIP

硫素水平对韭菜硝酸盐累积和氮代谢关键酶活性的影响 　　摘要：以日光温室多年生韭菜新世纪雪韭为试材，研究了土壤不同硫磺（S）施用量对韭菜硝酸盐含量、氮代谢关键酶活性和叶绿素荧光动力学参数的调控效应。结果表明，随着硫磺施用量的增加，韭菜叶片硝酸盐含量呈先减少后增加的趋势，当硫磺施用量为7.2 kg/hm2时硝酸盐含量最低，较对照减少7.2%；韭菜产量、硝酸还原酶（NR）活性、PSII最大光化学量子产量（Fv/Fm）、电子传递速率（ETR）和叶绿素含量则表现出先增加后降低的趋势，均以硫磺施用量7.2 kg/hm2时达到最大值，其中NR活性和ETR分别比对照提高了10.7%和25.8%；另外，谷氨酸丙酮酸转氨酶（GPT）和谷氨酸草酰乙酸转氨酶（GOT）活性随硫磺施用量的增加呈逐渐增加的趋势，硫磺施用量为54.0 kg/hm2时，GPT和GOT的活性达到最高，分别比对照增加了22.5%和9.4%。 　　关键词：硫磺；韭菜；硝酸盐累积；氮代谢关键酶 　　中图分类号：S633.3；S147.2 文献标识码：A 文章编号：0439-8114（2013）20-4882-04 　　Effects of Sulphur Levels on Nitrate Accumulation and Nitrogen Metabolism Activities of Key Enzymes in the Leaves of Chinese Chive 　　XIE Xin1，DUAN Li-xiao2，WANG Jun-ling3，GAO Zhi-kui1 　　（1. College of Horticulture， Agricultural University of Hebei，Baoding 071001，Hebei，China；2. Baoding Institute of Strawberries， Baoding 071000，Hebei，China；3. College of Life Sciences， Agricultural University of Hebei，Baoding 071001，Hebei，China） 　　Abstracts： The regulatory effects of soil concentration of sulfur processing on nitrate content， nitrogen metabolism activities of key enzymes and chlorophyll fluorescence parameters in the leaves of Chinese chive were studied in sunlight greenhouse. The results showed that the nitrate content first decreased and then increased with the increase in the sulfur concentration. When the sulfur concentration was 7.2 kg/hm2， the nitrate contend was the lowest and then decreased by 7.2% compared with the control. The production， nitrate reductase（NR） activity， PSII maximum quantum yield （Fv/Fm）， electron transport rate（ETR） and chlorophyll content of Chinese chive showed a decreasing trend after the first increase. The maximum was in sulfur concentration of 7.2 kg/hm2 comparing with the control. NRA and ETR increased 10.7% and 25.8%， respectively. In addition， glutamate pyruvate transaminase（GPT） and glutamate oxaloacetate transaminase（GOT） activity showed a gradual increase trend with the increase of S concentration. When concentration of S was 54.0 kg/hm2， GPT an