多药耐药相关蛋白在人肝细胞癌亚群细胞中的表达及意义.docVIP

多药耐药相关蛋白在人肝细胞癌亚群细胞中的表达及意义 　　作者：颜政 方驰华 作者单位：(1.青海大学附属医院肿瘤外科;2.南方医科大学珠江医院肝胆外科) 　　【摘要】 目的 检测肝细胞癌异质性亚群细胞对化疗药物的耐药差异，探讨耐药蛋白在肝癌组织中不同亚群细胞的表达意义。方法 ①通过MTT法分别测定人肝细胞癌异质性亚群细胞在不同浓度、不同化疗药物(ADM、VP-16、DDP)作用下的抑制率，计算每种药物IC50值。② 应用流式细胞仪分别检测耐药相关蛋白MDR1、MRP、LRP、GST在两亚群细胞中的表达率。结果 ①LCSC-1和LCSC-2亚群细胞对阿霉素(ADM)、顺铂(DDP)、鬼臼乙叉苷(VP-16)等药物都产生了耐药性(F=249.651)。三种药物对LCSC-2亚群细胞和LCSC-1亚群细胞抑制率有显著差异 (P0 .01)。②LCSC-1和LCSC-1亚群细胞MDR1、MRP、LRP、GST的表达量分别是：(23.97± 0.34)% 、(17.03±0.59)% 、(24.02±0.63)% 、(23.93± 0.59)% ;(26.59± 0.78)% 、(19.43± 0.60)% 、(26.43±1.06)% 、(26.24±1.06)% ;两亚群比较，t=5.32 ，P=0.006;t=4.94 ，P=0.008;t=3.85，P=0.028;t=3.30，P=0.030。两亚群细胞MDR1、MRP、LRP、GST的表达有显著性差异。结论 MRP、LRP、GST、MDR1的高表达，可能是人肝癌细胞异质性亚群多耐药产生的部分分子基础，耐药是肝细胞癌干细胞的特征之一。 　　【关键词】 肝细胞癌 异质性亚群细胞 多药耐药 　　EXPRESSION AND SIGNIFICANCE OF THE MULTIDRUG 　　RESISTANCE-ASSOCIATED PROTEIN IN SUBPOPULATION 　　CELL OF PRIMARY HEPATOCELLULAR CARCINOMAS 　　Yan Zheng1, Fang ChiHua2 　　(1.Department of Tumor Surgery, Qinghai University Affiliated Hospital ; 　　2.Department of General Surgery, Zhujiang Hospital, The Southern Medical University) 　　Abstract Objective To study the expression and significance of multidrug resistance-associated protein in human HCC heterogeneous subpopulations cells. Methods ①Resistance of subpopulations cells (LCSC-1 and LCSC-2) to different chemotherapeutic drugs (ADM, VP-16, and DDP) and the IC50 values of each drug are measured using MTT method. ②Number of positive cells of MDR1, MRP, LRP, GST related to drug resistance are measured using flow cytometry, and the average level of expression of drug-resistant proteins of each subpopulations cells is calculated. Results ①Both subpopulations showed resistance against ADM, DDP, and VP-16.The IC50 values of these drugs to LCSC-2 are higher than those to LSCS-1(F=249.651).This result suggests a higher drug resistance of LCSC-2 subpopulation (P0 .01). According to the results of statistical analysis, the inhibition rate of three drugs against LCSC-2 and L