Chondrogenic Differentiation of hMSCs on PCL Nanofibers对人骨髓间充质干细胞向软骨细胞分化的PCL纳米纤维.ppt

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Chondrogenic Differentiation of hMSCs on PCL Nanofibers对人骨髓间充质干细胞向软骨细胞分化的PCL纳米纤维.ppt

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8/3/2006 Chondrogenic Differentiation of hMSCs on PCL Nanofibers Winnie Kuo University of California, Berkeley Final Presentation for NSF-REU at UIC August 3, 2006 Advisors: Prof. Cho, Prof. Megaridis, Joel Wise Background Human Mesenchymal Stem cells (hMSCs) can differentiate into many cell lineages Chondrogenesis -- cartilage repair therapy Electrospun PCL nanofibrous scaffolds are biodegradable mimic extracellular matrix Goals Mimic thin superficial layer of articular (joint cartilage) Attach Differentiate hMSCs into cartilage cells on polymer nanofiber scaffolds Observe cell morphology differentiation based on physical cues Project Design Seed hMSCs on nanofibrous scaffolds Cultured with chondrogenic media in 96-well plates (control with growth media) Monitor cell proliferation differentiation: Fluorescence imaging Total DNA count Sulfated Glycosaminoglycan (sGAG) Cell Morphology Tracking Differentiation Conclusions Cells on nanofibers proliferate in an oriented manner Chondrogenic media and fiber alignment induce chondrogenesis By 5th week, chondrogenic cells produced high amounts of sGAG Oblong chondrogenic cell shape resembles superficial layer of articular cartilage Future Directions hMSCs cultured on nanofibers as an alternative source of cartilage cells Advantage: “renewable” Incorporate cartilage-inducing factors within nanofibers Chemicals proteins contained within fibers may mimic ECM better than mere suspension Acknowledgements NSF EEC-0453432 Grant Novel Materials and Processing in Chemical and Biomedical Engineering (Director C.G. Takoudis) Funded by the DoD-ASSURE and NSF-REU Programs Professor M. Cho Professor C. Megaridis Professor A. Yarin Joel Wise References Reneker, D.H. et al, Electrospinning of Nanofibers from Polymer Solutions and Melts, Advances in Applied Mechanics, Vol. 41, Elsevier Inc. 2006. Tuan, R.S., Song, L., Baksh, D., Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therap