透骨消痛颗粒干预OA早期COX-2和iNOS表达的实验研究.doc

透骨消痛颗粒干预OA早期COX-2和iNOS表达的实验研究 高雪伟 摘 要：目的 通过动物试验探讨透骨消痛颗粒对骨性关节炎早期环氧化酶-2 (COX-2)和 诱导性一氧化氮合酶(iNOS)表达的影响，研究透骨消痛颗粒在膝骨性关节炎治疗中的相关作用机制。方法 新西兰大白兔采用改良Hulth法造模，造模成功后随机分成3组：模型组、对照组、实验组,和正常组共4组，分别灌胃给药4周后处死动物取材并观测如下指标：关节腔大体观察，滑膜与软骨组织学观察；关节液内NO、NOS、PGE2含量检测，滑膜软骨中iNOS、COX-2含量检测。结果 4周后关节液中NO、NOS含量、PGE2浓度，滑膜和软骨中iNOS、COX-2含量，模型组、对照组、实验组均呈高表达，与正常组比较差异有显著性（P〈0．05）；对照组、实验组与模型组比较差异有显著性（P〈0．05）；对照组与实验组比较差异有显著性（P〈0．05）。结论 透骨消痛颗粒可通过抑制COX-2和iNOS在OA早期滑膜和软骨细胞中的表达及其诱导产物PGE2和NO的生成，从而达到控制OA早期炎症、减轻疼痛、缓解症状的作用 关键词：透骨消痛颗粒 骨性关节炎 滑膜 软骨 COX-2 iNOS NO PGE2 The Experimental Research about granula of penetrating bone removeing pain intervene the expression of COX-2 and iNOS in early stage of osteoarthritis Xuewei Gao，Jinshui Zhou，Boling Liu Abstract Objective: To investgate the mechanism of influence about granula of penetrating bone removeing pain intervene the expression of COX-2 and iNOS in early stage of osteoarthritis by animals experiment, to investgate mechanism of granula of penetrating bone removeing pain on treatment of osteoarthritis .Methods:We devided the New Zealand rabbits into 3 groups randomly after replicated knee osteoarthritis with modified Hulth modeling method:model group ,control group , experimental group, with the normal group were 4 groups in total. we execute all rabbitsafter they were fed intragastically for 4weekes. Then the following detection were taken out: General Observation of articular cavity; histological Conversation of synovium and cartilage; content of NOS , NO , test of iNOSmRNA, concentration of COX-2 and PGE2 within joint . Results:After four weekes, there was significant contrast(P0.05) of the concent of NOS , NO, iNOSmRNA, COX-2 and PGE2 of model group ,control group , experimental group compared with the normal group; There was significant contrast(P0.05) of the concent of NOS , NO, iNOSmRNA, COX-2 and PGE2 of experimental groups and control group compared with the model group; There was significant contrast(P0.05) of the concent of NOS , NO, iNOSmRNA, C