experiment6- anoka- ramsey community college（experiment6 -安诺卡-拉姆齐社区学院）.doc

Lab Activity H5 Paper Chromatography of MM’s OUTCOMES After completing this lab activity, the student should be able to: explain basic principles of chromatography in general. describe important aspects of paper chromatography. identify the number and nature of the components found in different color MM’s. DISCUSSION Chromatography is one of the most important and widely used analytical techniques known to chemists. It is a technique that is used for separating, purifying, and identifying certain chemical compounds. Chromatography means literally, written in color, since it was a technique originally used to separate colored materials, like the pigments in flowers. Today it is used to separate very complex mixtures, often containing several hundred compounds. Modern instruments are equipped with various sensitive detectors that allow chromatographic techniques to be carried out with colorless compounds, often with very minute quantities. Chromatography is used in the separation of petroleum, natural and artificial flavorings, amino acids, perfumes, and many others. It is also used to identify components in these mixtures or drugs that may be present in a urine or plasma sample. There are many different types of chromatographic techniques used today. These include high-pressure liquid chromatography (HPLC), gas chromatography (GC), column chromatography, thin-layer chromatography (TLC), and the technique you will be using in this experiment — paper chromatography. All types of chromatography involve a stationary phase and a mobile phase. In paper chromatography, the substance to be analyzed is dissolved in a solvent. The resulting solution is then spotted onto a rectangular piece of filter paper, near the bottom, and allowed to dry. The paper is then lowered into a sealed chamber containing a small amount of a solvent or mixture of solvents, keeping the spots above the surface of the solvent. Once lowered into the chamber, the solvent begins to wick