头孢他啶与中药制剂联合应用对铜绿假单胞菌的体外抗菌活性的研究.docVIP

头孢他啶与中药制剂联合应用对铜绿假单胞菌的体外抗菌活性的研究 　　摘要：目的 研究头孢他啶和双黄连注射液、热毒宁注射液、喜炎平注射液联合应用抗临床分离的铜绿假单胞菌的效果。方法 收集并分离临床来源的68株铜绿假单胞菌，采用微量棋盘稀释法，计算头孢他啶分别和3种中药制剂的FIC指数。结果 采用头孢他啶和热毒宁注射液联用，以相加（11.76%）和无关（58.82%）作用为主，以协同（10.29%）和拮抗（8.82%%）为次。采用头孢他啶和双黄连注射液联用，以无关（36.76%）和相加（20.59%）作用为主，以协同（11.76%）和拮抗（16.18%）为次。头孢他啶与喜炎平注射液联用抗铜绿假单胞菌无效。结论 在临床治疗过程中，可以佐以中药制剂以增强抗菌药物治疗的有效性。 　　关键词：铜绿假单胞菌；联合用药；微量棋盘稀释法 　　Study on Antibacterial Activity of Ceftazidime with Traditional Chinese Medicine Preparation Combined Application of Pseudomonas Aeruginosa in Vitro 　　LV Juan， XIE Fen，DING Yong-juan 　　（Affiliated Hospital of Jiangnan University， Wuxi N0’4 Peoples Hospital， Wuxi 214062，Jiangsu，China） 　　Abstract： Objective To study the effect of antibacterial action combined with ceftazidime and Shuanghuanglian injection， Reduning injection， Xiyanping injection on Pseudomonas Aeruginosa. Methods To collect and isolate the 68 strains of Pseudomonas Aeruginosa in clinical， the fractional inhibitory concentration index was calculated with microdilution checkerboard method， about ceftazidime and Shuanghuanglian injection， Reduning Injection， Xiyanping Injection. Results The effect of antibiotics combination of ceftazidime and Reduning Injection was 11.76% additive and 58.82% independent action， while 10.29% was synergistic effect and 8.82% was antagonistic effect. The effect of antibiotics combination of ceftazidime and Shuanghuanglian Injection was 20.59% additive and 36.76% independent action， while 11.76% was synergistic effect and 16.18% was antagonistic effect. It is ineffective for us to combine ceftazidime with Xiyanping injection. Conclusion In the clinical course of treatment， chinese medicine preparation can be used to enhance the effectiveness of antimicrobial therapy. 　　Key words：Pseudomonas aeruginosa；Combination；Microdilution checkerboard method 　　铜绿假单胞菌（Pseudomonas aeruginosa，PA）是假单胞菌中最常见的条件致病菌，是我院培养分离出的前三个病原菌之一。PA感染难以治疗，甚而产生多重耐药、泛耐药菌株，有相当部分原因是因为铜绿假单胞菌的耐药机制十分复杂。当单一抗菌药物的使用无法有效的治疗铜绿假单胞菌感染时，临床通常选择不同作用机制的抗