骨髓增生异常综合征患者血管新生相关因子血清水平研究.docVIP

骨髓增生异常综合征患者血管新生相关因子血清水平研究 【摘要】 　　为了研究骨髓增生异常综合征（MDS）患者血清血管内皮生长因子（VEGF）及碱性成纤维细胞生长因子（bFGF）的水平，应用酶联免疫吸附（ELISA）实验检测43例MDS患者的血清。结果表明，MDS患者VEGF、bFGF血清水平均明显高于正常对照，RAEB、RAEBT组均明显高于RA、RAS组，RAEBT组与急性髓细胞白血病（AML）组VEGF、bFGF水平无显著差别。VEGF、bFGF水平与患者的外周血细胞受累、骨髓原始细胞比例有关，VEGF与bFGF正相关（r＝0.539，P＜0.01）。结论：MDS患者VEGF、bFGF增多，其水平与MDS类型及预后有关。 【关键词】 骨髓增生异常综合征　血管内皮生长因子　碱性成纤维细胞生长因子 　　Serum Level of Angiogenesisrelated Cytokins in Patients with Myelo dysplastic Syndrome Abstract To study the serum levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in patients with myelodysplastic syndrome (MDS), serum from 43 MDS patients was examined by enzyme linked immunosorbent assay (ELISA).The results showed that serum levels of VEGF, bFGF in MDS patients were significantly elevated compared with normal control. Serum levels of VEGF and bFGF in RAEB patients and RAEBT patients were higher than that in RA patients and RAS patients （P＜0.05). No significant difference of serum levels of VEGF and bFGF was found between RAEBT patients and acute myeloid leukemia (AML) patients. The serum levels of VEGF and bFGF were correlated with the affected peripheral blood cells and the proportion of blast cells in bone marrow. There was positive correlation between serum VEGF and bFGF levels. It is concluded that the secretion of VEGF and bFGF in MDS patients is elevated and the serum levels of VEGF and bFGF are related to the classification and prognosis in MDS. Key words myelodysplastic syndrome; vascular endothelial growth factor; basic fibroblast growth factor 近年来研究表明, 血管新生及其调控因子在血液系统恶性肿瘤的发生发展中起重要作用。血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）是目前所知的最重要的血管新生正调控因子，可以反映骨髓微血管增生的程度，在多种恶性血液疾病中表达增高。关于骨髓增生异常综合征（MDS）患者VEGF、bFGF表达的研究，目前国内罕见有报道。为此，我们检测了43例MDS患者的血清VEGF 和bFGF水平，现报道如下。 材料和方法 研究对象 43例MDS患者均为青岛大学医学院附属医院血液内科2004年8月份至2005年8月份门诊或住院的初诊病人，其中男性23 例，女性20 例，年龄23-76岁，中位年龄52岁。43例MDS患者中RA 16例，RAS 6例，RAEB 14例，RAEBT 7例。15例健康体检者为正常对照，15例初诊AML患者为疾病对照