芥菜中总黄酮的提取及其对羟自由基的清除作用研究.docVIP

芥菜中总黄酮的提取及其对羟自由基的清除作用研究 【摘要】 目的探讨芥菜中总黄酮的提取、鉴别方法及对羟自由基清除作用，以充分利用芥菜植物资源，避免资源的浪费。方法采用超声波乙醇浸提法从芥菜中提取黄酮类物质，对所提取的黄酮类物质进行验证，并用分光光度法测定含量，用芥菜总黄酮对羟自由基清除作用进行实验。结果测得样品中总黄酮的含量C=0.4450 mg/ml，回收率为100.7%，其纯度和产率均较高。结论该方法采用全物理过程，无任何污染，是提取芥菜中黄酮类物质的有效途径。芥菜总黄酮提取液对Fenton体系产生的·OH自由基有很好的清除作用。 　　 【关键词】 超声波提取 芥菜 总黄酮 鉴别 自由基 清除 　　Extraction of Total Flavone from Bragica juncea Coss and Its Effects on Scavenging of Hydroxyl Radicals 　　Abstract：ObjectiveTo make use of the resources of Bragica juncea coss， avoid wasting and approach the extraction of total flavanone of Bragica juncea Coss and its effects on scavenging of hydroxyl radicals. MethodsThe flavonoids were extracted by ethanol as the solvent from Bragica juncea coss with ultrasonic wave and ethanol extraction .Using spectrophotometry to extract and check the flavone of Bragica juncea Coss.Results The effect of extracts of Bragica juncea Coss scavenging of hydroxyl radicals was studied．By this method getting the density of the total flavone of Bragica juncea Coss was C=0.4450mg/ml and the rate of recovery was 100.7%. The outcome and the purity of the flavone were all very high . ConclusionThis method is a purely physical process and has not any pollution. It is an ideal way to extract the flavone of Bragica juncea coss. Total flavone from Bragica juncea Coss has effects on scavenging on hydroxyl radicals. 　　Key words：Ultrasonic wave treatment； Bragica juncea Coss； Total flavone； Identification； Hydroxyl radical； Scavenging 芥菜 Bragica juncea Coss又名芥、雪里蕻、皱叶芥、黄芥，为十字花科植物芥菜 Brassia juncea（L.）Czern.et Coss油芥菜Brassia juncea（L.）Czern.et Coss.Var.gracilis Tsen et Lee的嫩茎叶。我国各地均有栽培。春季采嫩叶洗净，民间常用之做腌菜。芥菜性味辛、温。归肺、胃、肾经。具有利气豁痰、温中健脾、驱风散血、消肿止痛等功效。主治寒痰咳嗽、胸膈不利、胃寒少食、腹泻、感冒风寒及跌打损伤、关节疼痛等症 ［1］。目前有研究表明［2］：芥菜中含黄酮苷、酸性树脂、鞣质等，其中黄酮类等有效成分对治疗冠心病、老年性痴呆、脑血栓、神经系统疾病和清除自由基、抑菌、抗癌等方面有显著效果，无毒副作用，并以此开发出多种药品和保健食品。 当前国内外对于芥菜中黄酮类化合物的研究还相对缺乏。目前有用乙醇抽提法提取芥菜中的黄酮类物质的报道［3］，但是，该方法提取的时间较长、工艺较复杂、提取效率较低。近年来，超声技术应用于提取植物中的生物碱、苷类、生物活性物质、动物组织浆的毒质等