金纳多对抗大鼠脑出血后脑水肿的作用机制.docVIP

金纳多对抗大鼠脑出血后脑水肿的作用机制 作者：杨霄鹏，李秋芳，杨瑞玲 【摘要】 目的 探讨金纳多对抗脑出血后脑水肿形成的作用机制。方法 选健康Wistar大鼠80只，雌雄不拘，随机分为4组，每组20只。采用立体定向注射方法，用微量注射器在右侧尾状核部位，注入50μL生理盐水(A、B组)或等量自体血红蛋白(C、D组)。术后A、C组给予50g/L羧甲基纤维素钠溶液3mL灌胃，B、D组给予等量羧甲基纤维素钠溶解均匀的金纳多(200mg/kg)溶液灌胃，每日1次，持续3周。断头取脑，测定超氧化物歧化酶(SOD)、丙二醛(MDA)值及血红素氧合酶1(HO1)阳性细胞数。结果 除A、B组比较无明显差异外，其余各组间SOD、MDA含量及HO1阳性细胞数比较差异均有统计学意义(Plt;0.05)。结论 金纳多可以抑制血红蛋白所引起的脑水肿。 【关键词】 金纳多；脑出血；脑水肿；超氧化物歧化酶；丙二醛；血红素氧合酶1 　　Rivalry mechanism of ginkgo in rat brain edema　after intracerebral hemorrhage 　　ABSTRACT: Objective To investigate the rivalry mechanism of ginkgo in rat brain edema after intracerebral hemorrhage. Methods Totally 80 healthy Wistar rats were divided into 4 groups randomly (20 rats in each group). Fifty microliters saline (group A and group B) or autohemoglobin (group C and group D) was infused into the right caudate nuclears using stereo tactic guidance. Groups A and C were lavaged 50g/L carboxymethyl cellulose sodium solution of 3mL, groups B and D were lavaged the same amount of ginkgo (200mg/kg) solution dissolved by 50g/L carboxymethyl cellulose sodium solution once a day for 3 weeks. All the rats were decapitated after raised for 3 weeks, and superoxide dismutase, malondialdehyde, and the number of hemeoxygenase1 (HO1) positive cells in brains were assayed. Results The differences in SOD and MDA contents as well as HO1 positive cell number between every two groups were significant (Plt;0.05) except group A and group B. Conclusion Ginkgo can inhibit brain edema after intracerebral hemorrhage caused by hemoglobin. 　　KEY WORDS: ginkgo; cerebral hemorrhage; brain edema; superoxide dismutase; malondialdehyde ; hemeoxygenase1 　　红细胞自身溶解后释放的血红蛋白主要通过血红素氧合酶1(hemeoxygenase1, HO1)进行降解，其降解产物可通过多种途径引起脑水肿。金纳多是一种标准化银杏叶萃取物，能改善缺血脑组织的能量代谢，保护细胞膜的结构和功能的完整性，对缺血神经细胞有保护作用[12]。本研究以脑组织匀浆中超氧化物歧化酶(superoxide dismutase, SOD)活性、丙二醛(malondialdehyde, MDA)值及免疫组化HO1为指标，观察金纳多对大鼠脑出血后脑水肿的保护作用，旨在为其广泛应用于临床防治脑出血后脑水肿提供理论依据。 　　1 材料与方法 　　1.1