金雀异黄素对高糖培养下大鼠系膜细胞增殖及凋亡的影响.docVIP

金雀异黄素对高糖培养下大鼠系膜细胞增殖及凋亡的影响 作者：孙莉静，袁伟杰，张小瑛，梅小斌，郭志勇，陈光椿，张军 【关键词】 细胞凋亡 Effect of genistein on apoptosis and proliferation of rat mesangial cells cultured in high glucose 　　【Abstract】 AIM: To evaluate the effect of different concentrations of genistein on the proliferation and apoptosis of rat mesangial cells cultured in vitro in high glucose. METHODS: Mesangial cells were treated in different groups. Control group (low glucose 1 g/L without genistein) and experimental groups (high glucose 4.5 g/L with genistein 0, 1, 5, 10, 30 and 50 μmol/L) were established and incubated for 12, 24, 36 and 48 h. The proliferation effect of genistein on cells was measured by MTT method. Agarose gel electrophoresis of DNA extracted from different groups, the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) technique and flow cytometry using propidium iodide staining were performed to identify or quantify the apoptosis of cells. RESULTS: Quantitative analysis of apoptotic cells showed that the apoptotic rate of the mesangial cells treated with genistein for 24 h was significantly higher, in 10, 30 and 50 μmol/L genistein groups than that in normal control group (Plt;0.05). No significant difference of the growth inhibition was found in 0, 1 and 5 μmol/L genistein groups. The cell apoptotic rate increased in a positive manner of genistein concentration and culture duration. CONCLUSION: Genistein has an intrinsic capability of inhibiting the proliferation and inducing the apoptosis of mesangial cells in cultured high glucose and these effects are in a genistein concentration and time dependent manners. 　　【Keywords】 genistein；cell proliferation；apoptosis 　　【摘要】 目的：探讨金雀异黄素对体外高糖条件下大鼠系膜细胞（MC）增殖及凋亡的影响. 方法：对照组：低糖DMEM培养液（不含金雀异黄素），实验组：高糖DMEM培养液（含0，1,5，10，30，50 μmol/L金雀异黄素），各组分别培养12，24，36，48 h. 氮蓝四唑盐（MTT）法检测MC的增殖情况，DNA琼脂糖电泳、末端转移酶介导的dUTP缺口末端标记（TUNEL）法、流式细胞术3种方法检测细胞凋亡情况. 结果：10，30，50 μmol/L金雀异黄素作用24 h可明显诱导细胞凋亡（Plt;0.05），而0，1，5