菊花脑保肝作用的有效部位筛选.docVIP

菊花脑保肝作用的有效部位筛选 作者：任爱农，刘春宇，杨念云 【摘要】 目的 在菊花脑不同极性提取部位(CFDN)中筛选出保肝作用的有效部位。方法 采用0.1%CCl4(10ml/kg)ip小鼠，造成急性肝损伤的模型，测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)，观察肝组织病变；用CCl4损伤大鼠肝细胞，MTT法测定肝细胞增殖活性。结果 CFDN的60%乙醇和95%乙醇柱洗脱部位能显著降低小鼠CCl4肝损伤血清中ALT和AST的活性(P＜0.01，P＜0.05)，病理学观察显示：该部位能明显减轻肝损伤的程度(P＜0.01，P＜0.05)，CFDN的60%乙醇和95%乙醇柱洗脱部位能显著提高肝细胞活性，促进肝细胞增殖(P＜0.01)。结论 CFDN的60%乙醇和95%乙醇柱洗脱部位具有显著的保肝作用。 【关键词】 菊花脑；有效部位；CCl4；肝损伤 Screening of the active fractions from Chrysanthemum nankingense on their liver-protective effects 【Abstract】 Objective To screen the active fractions from Dendranthema Nankingense of Chrysanthemum nankingense on their liver-protective effects.Methods The mouse models of carbon tetrachloride-induced acute hepatic injury were employed in this study.The activity of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum were assaied.Also pathological changes were observed in liver tissues.The proliferous activities were assaied by MTT in cells of liver-injured rat by 0.1%CCl4(10ml/kg)ip.Results The CFDN of 60% alchohol and 95% alchohol can debase ALT and AST activities in the serum of liver-injuried mice(P＜0.01，P＜0.05).This can elevate the activities of liver cells and accelerate hepatocellular proliferation.The pathological observation indicated the degrees of inflammatory activity in liver tissues were markedly reduced(P＜0.01).Conclusion The results show that CFDN of 60% alchohol and 95% alchohol have highly antihepatotoxic effect. 【Key words】 Chrysanthemum nankingense；effective site;carbon tetrachloride；hepatic injury 菊花脑系菊科植物菊花脑(Chrysanthemum nankingense Hand-Mezz)的干燥嫩茎叶，为江苏地产资源，其味苦、辛，性凉；功能：清热解毒。该品种于2002年在江苏省科技厅自然基金立项，经研究，从该植物中提取分离得到12个化合物，主要为二十六烷酸、谷甾醇、金圣草黄素、木犀草素等，其中木犀草素是黄酮类的代表性成分［1］。该植物的不同极性部位经体内外抑菌、消炎、抗病毒的试验均显示出相应的活性部位。在此基础上我们对其保肝作用进行了探讨。采用0.1%CCl4(10ml/kg)ip小鼠，造成急性肝损伤的模型，测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)，观察肝组织病变以及用CCl4损伤大鼠肝细胞，MTT法测定肝细胞增殖活性，观察菊花脑不同极性部位(CFDN)的保肝作用。 1 材料 1.1 药物 菊花脑采集于南京老山，原植物经南京中医药大学刘训红教授鉴定为菊花脑(Chrysanthem