非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化.docVIP

非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化 武海军1，银和平2，胡继平1，刘 聪1，李树文2，白 明2，杜志才2(1呼和浩特市第一医院，内蒙古自治区呼和浩特市 010010； 引用本文：武海军，银和平，胡继平，刘聪，李树文，白明，杜志才.非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化[J].中国组织工程研究，2016，20(45):6706-6713. DOI: 10.3969/j.issn.2095-4344.2016.45.003 ORCID: 0000-0001-6607-7156(银和平) 文章快速阅读： 文题释义： Transwell 小室共培养实验4 μm，相Effect of indirect co-culture system on differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells Wu Hai-jun1, Yin He-ping2, Hu Ji-ping1, Liu Cong1, Li Shu-wen2, Bai Ming2, Du Zhi-cai2 (1First Hospital of Hohhot, Hohhot 010010, Inner Mongolia Autonomous Region, China; 2the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China) Abstract BACKGROUND: Mesenchymal stem cells (MSCs) for the treatment of degenerative disc diseases present a new therapeutic strategy for the structural and functional recovery of the degenerative intervertebral disc. OBJECTIVE: To study the differentiation potential of rabbit bone marrow MSCs (BMSCs) into nucleus pulposus-like cells. METHODS: Passage 3 BMSCs and nucleus pulosus cells (NPCs) extracted from rabbits were assigned into simple BMSCs culture, simple NPCs culture or co-culture group. In the former two groups, BMSCs or NPCs were cultured in 6-well culture plates. In the co-culture group, passage 3 BMSCs and NPCs were cultured on the upper or lower layer of the 6-well Transwell chamber, respectively. Cell morphology was observed by inverted contrast phase microscope. At 7 days of culture, immunocytochemistry, RT-PCR and western blot were used to examine the expression levels of type II collagen and aggrecan. RESULTS AND CONCLUSION: At 7 days of co-culture, BMSCs were polygonal or short spindle-shaped, with no fiber-like changes, and cell morphology was close to that of NPCs. Additionally, the expression levels of type II collagen and aggrecan in the BMSCs co-cultured with NPCs were similar to those in the NPCs cu