不同地区桔梗多糖含量的比较.doc

不同地区桔梗多糖含量的比较张小斌 （商洛学院生物医药工程系、中国中医研究院商洛中药材GAP科研工程中心 陕西商洛 726000） 摘要 选取陕西商洛、安徽太和和吉林延边产桔梗，采用水提取法提取桔梗粗多糖，分别测定粗多糖含量，采用三氯乙酸－正丁醇法脱蛋白脱去多糖中粗蛋白，测定精制多糖含量，比较三个地区桔梗粗多糖及精制多糖含量。结果显示，安徽太和粗多糖含量最高，为26.36%，其次为吉林延边桔梗，为18.27%，陕西商洛桔梗为17.08%。提取粗多糖经精制后，安徽太和、陕西商洛、吉林延边桔梗粗多糖蛋白去除率分别为：74.27%，68.08%和69.36%，差异不显著。精制多糖含量分别为：12.61%，15.42%和11.42%。结果表明安徽太和桔梗多糖含量最高，陕西商洛次之，最后为吉林延边桔梗。 关键词：桔梗；粗多糖；精制多糖；含量 中图分类号： 文献标识码： 文章编号： Comparison the content of polysaccharide of platycodon in three areas Abstract: Select the platycodon,in shangluo, anhui tai and jilin in yanbian with water to extrac the crude polysaccharide of platycodon,ahd then crude polysaccharide is determined,Use the acid-n-buoh the different method to take off the protein in the polysaccharide crude, and then to determine the refined polysaccharide, compare the thick polysaccharides and refined polysaccharide of platycodon in these three areas.The result is showed that the highest content of polysaccharide in anhui tai, thick for 26.36%, followed by jilin yanbian balloon flower18.27%, the minimum is shangluo balloonflower, for 17.08%..After the extraction of refined polysaccharide from crude polysaccharide,the balloonflower thick glycoprotein removal rate in anhui tai, shangluo, jilin yanbian is more than respectively 74.27%,68.08% and 69.36%, the difference is not significant. Refined polysaccharide is 12.61%，15.42% and 11.42%, the results show that the contents of polysaccharide in anhui tai balloonflower highest, in shangluo is the second smallest, and finally for jilin yanbian lisianthus. Key words: platycodon; crude polysaccharide; Refined polysaccharide; content 1.1选题的目的 桔梗[1](Platycodon grandiflorum(Jacq．)A．DC．)是桔梗科桔梗属、多年生草本植物, 别名铃铛花、梗草、和尚头花、土人参、包袱花等。药用其根, 为桔梗科植物桔梗的干燥根。我国最早药书 《神农本草经》 有记载, 桔梗系常用中药桔梗花大美丽，可供观赏，种子可榨油，根是一种常见的中药材。其味苦、辛，性微温有宣肺、散寒、祛痰、排脓等功效，主治外感咳嗽、喉痹咽痛、胸满肋痛等症。现代医学认为，桔梗富含桔梗皂甙、多糖、甾醇类、萜类、桔梗酸等成分，并含有十几种氨基酸及铜、铁、锌、锰等22种微量元素，具有抗炎、止咳、化痰、抗溃疡、扩张血管、降血压、解热镇痛、镇静、降血糖、促进胆酸分泌、抗过敏等许多药理及保健功能[2]。除此以外，桔梗还可以作为蔬菜食用，在